Trace element mapping of a single cell using a hard x-ray nanobeam focused by a Kirkpatrick-Baez mirror system

被引：46

作者：

Matsuyama, S. ^{[1
]}

Shimura, M. ^{[2
]}

Mirnura, H. ^{[1
]}

Fujii, M. ^{[1
]}

Yumoto, H. ^{[3
]}

Sano, Y. ^{[1
]}

Yabashi, M. ^{[3
]}

Nishino, Y. ^{[4
]}

Tamasaku, K. ^{[4
]}

Ishikawa, T. ^{[4
]}

Yamauchi, K. ^{[1
,5
]}

机构：

[1] Osaka Univ, Dept Precis Sci & Technol, Grad Sch Engn, Suita, Osaka 5650871, Japan

[2] Int Med Ctr Japan, Dept Intractable Dis, Shinjuku Ku, Tokyo 1628655, Japan

[3] Spring 8 Synchrotron Radiat Res Inst JASRI, Sayo, Hyogo 6795148, Japan

[4] Spring 8 Riken, Sayo, Hyogo 6795148, Japan

[5] Osaka Univ, Res Ctr Ultra Precis Sci & Technol, Grad Sch Engn, Suita, Osaka 5650871, Japan

来源：

X-RAY SPECTROMETRY | 2009年 / 38卷 / 02期

关键词：

CIS-DIAMMINEDICHLORO-PLATINUM(II); LOCALIZATION; MICROSCOPY;

D O I：

10.1002/xrs.1123

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

To visualize the distributions of trace elements in biological samples such as tissues and cells at high spatial resolution, we developed a scanning x-ray fluorescence microscope (SXFM) at SPring-8, using a Kirkpatrick-Baez mirror optics that enables achromatic and highly efficient focusing. To evaluate performance regarding its application to biological samples, the SXFM was used at x-ray energy of 15 keV to observe NIH/3T3 cells in which adenosine triphosphate (ATP) synthase beta (specifically localized at the mitochondria) were labeled with gold colloidal particles. Various elemental distributions were visualized at the single-cell level, including those for P, S, Cl, Ca, Fe, Cu, Zn and Au, and we obtained high-resolution elemental distribution maps by magnifying the labeled single mitochondrion. Maximum spatial resolution achieved in the experiments was sub-100 nm. Copyright (C) 2008 John Wiley & Sons, Ltd.

引用

页码：89 / 94

页数：6

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