Characterization of a novel miniaturized burst-mode infrared laser system for IR-MALDESI mass spectrometry imaging

被引：32

作者：

Ekelof, Mans ^{[1
]}

Manni, Jeffrey, Sr. ^{[2
]}

Nazari, Milad ^{[1
]}

Bokhart, Mark ^{[1
]}

Muddiman, David C. ^{[1
,3
]}

机构：

[1] North Carolina State Univ, Dept Chem, FTMS Lab Human Hlth Res, Raleigh, NC 27695 USA

[2] JGM Associates Inc, 15 Dist Ave, Burlington, MA 01803 USA

[3] North Carolina State Univ, Mol Educ Technol & Res Innovat Ctr, Raleigh, NC 27695 USA

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2018年 / 410卷 / 09期

基金：

美国国家卫生研究院;

关键词：

Mass spectrometry imaging; Infrared laser; IR-MALDESI; HRAM mass spectrometry; ELECTROSPRAY-IONIZATION; ATMOSPHERIC-PRESSURE; ORGANIC-MOLECULES; TISSUE-SECTIONS; MATRIX; ABLATION;

D O I：

10.1007/s00216-018-0918-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Laser systems are widely used in mass spectrometry as sample probes and ionization sources. Mid-infrared lasers are particularly suitable for analysis of high water content samples such as animal and plant tissues, using water as a resonantly excited sacrificial matrix. Commercially available mid-IR lasers have historically been bulky and expensive due to cooling requirements. This work presents a novel air-cooled miniature mid-IR laser with adjustable burst-mode output and details an evaluation of its performance for mass spectrometry imaging. The miniature laser was found capable of generating sufficient energy for complete ablation of animal tissue in the context of an IR-MALDESI experiment with exogenously added ice matrix, yielding several hundred confident metabolite identifications.

引用

页码：2395 / 2402

页数：8

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