Enzymatic Synthesis of L-Ascorbyl Laurate in DMSO-Acetone Mixed Solvent

被引：11

作者：

Zhang, Dong-Hao ^{[1
,2
]}

Li, Chao ^{[1
]}

Xie, Ling-Ling ^{[1
]}

Yuwen, Li-Xia ^{[1
]}

机构：

[1] Hebei Univ, Coll Pharm, Baoding 071002, Peoples R China

[2] Hebei Univ, Coll Pharm, Key Lab Pharmaceut Qual Control Hebei Prov, Baoding 071002, Peoples R China

来源：

INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH | 2013年 / 52卷 / 34期

关键词：

IMMOBILIZED LIPASE; CINNAMIC ACID; ESTERIFICATION; BIOSYNTHESIS; ESTERS;

D O I：

10.1021/ie4016396

中图分类号：

TQ [化学工业];

学科分类号：

0817 ;

摘要：

In the present study, a dimethyl sulfoxide (DMSO)-acetone mixed solvent was used as a reaction medium to study lipase-catalyzed esterification of L-ascorbic acid with lauric acid as the acyl donor. Compared with the solvent of acetone, the mixed solvent not only could greatly improve the solubility of L-ascorbic acid but also could observably increase the enzymatic rate. Within the investigated range of substrate concentrations, a lauric acid/ascorbic acid mole ratio of 3:1 was found to be optimum. Moreover, the optimum production was achieved in the reaction medium dewatered by molecular sieves and at a lipase loading of 40 mg/rnL. As a result, enzymatic synthesis of ascorbyl laurate gave a maximum conversion of 94% in the DMSO-acetone mixed solvent using lauric acid as the acyl donor.

引用

页码：11875 / 11879

页数：5

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