A bioreducible linear poly(β-amino ester) for siRNA delivery

被引：68

作者：

Kozielski, Kristen L.

Tzeng, Stephany Y.

Green, Jordan J. ^{[1
]}

机构：

[1] Johns Hopkins Univ, Sch Med, Dept Biomed Engn, Dept Ophthalmol,Inst Nanobiotechnol, Baltimore, MD 21231 USA

来源：

CHEMICAL COMMUNICATIONS | 2013年 / 49卷 / 46期

基金：

美国国家科学基金会;

关键词：

NONVIRAL GENE DELIVERY; NANOPARTICLES; RNA; DNA; VECTORS; CELLS;

D O I：

10.1039/c3cc40718g

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Described here is the synthesis and characterization of a novel, bioreducible linear poly(beta-amino ester) designed to condense siRNA into nanoparticles and efficiently release it upon entering the cytoplasm. Delivery of siRNA using this polymer achieved near-complete knockdown of a fluorescent marker gene in primary human glioblastoma cells with no cytotoxicity.

引用

页码：5319 / 5321

页数：3

共 19 条

[1] The relationship between terminal functionalization and molecular weight of a gene delivery polymer and transfection efficacy in mammary epithelial 2-D cultures and 3-D organotypic cultures [J].

Bhise, Nupura S. ;

Gray, Ryan S. ;

Sunshine, Joel C. ;

Htet, Soe ;

Ewald, Andrew J. ;

Green, Jordan J. .

BIOMATERIALS, 2010, 31 (31) :8088-8096

[2] Mechanistic investigation of poly(ethylene imine)-based siRNA delivery: Disulfide bonds boost intracellular release of the cargo [J].

Breunig, Miriam ;

Hozsa, Constantin ;

Lungwitz, Uta ;

Watanabe, Kazuo ;

Umeda, Isao ;

Kato, Hiroyuki ;

Goepferich, Achim .

JOURNAL OF CONTROLLED RELEASE, 2008, 130 (01) :57-63

[3] pH and Reduction Dual-Sensitive Copolymeric Micelles for Intracellular Doxorubicin Delivery [J].

Chen, Jun ;

Qiu, Xiaozhong ;

Ouyang, Jun ;

Kong, Jiming ;

Zhong, Wen ;

Xing, Malcolm M. Q. .

BIOMACROMOLECULES, 2011, 12 (10) :3601-3611

[4] Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans [J].

Fire, A ;

Xu, SQ ;

Montgomery, MK ;

Kostas, SA ;

Driver, SE ;

Mello, CC .

NATURE, 1998, 391 (6669) :806-811

[5] A combinatorial polymer library approach yields insight into nonviral gene delivery [J].

Green, Jordan J. ;

Langer, Robert ;

Anderson, Daniel G. .

ACCOUNTS OF CHEMICAL RESEARCH, 2008, 41 (06) :749-759

[6] Biologic and pharmacologic regulation of mammalian glutathione synthesis [J].

Griffith, OW .

FREE RADICAL BIOLOGY AND MEDICINE, 1999, 27 (9-10) :922-935

[7] RNAi - Prospects for a general technique for determining gene function [J].

Kuwabara, PE ;

Coulson, A .

PARASITOLOGY TODAY, 2000, 16 (08) :347-349

[8] Gold, Poly(β-amino ester) Nanoparticles for Small Interfering RNA Delivery [J].

Lee, Jae-Seung ;

Green, Jordan J. ;

Love, Kevin T. ;

Sunshine, Joel ;

Langer, Robert ;

Anderson, Daniel G. .

NANO LETTERS, 2009, 9 (06) :2402-2406

[9] Degradable poly(β-amino esters):: Synthesis, characterization, and self-assembly with plasmid DNA [J].

Lynn, DM ;

Langer, R .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2000, 122 (44) :10761-10768

[10] Disulfide cross-linked polyethylenimines (PEI) prepared via thiolation of low molecular weight PEI as highly efficient gene vectors [J].

Peng, Qi ;

Zhong, Zhenlin ;

Zhuo, Renxi .

BIOCONJUGATE CHEMISTRY, 2008, 19 (02) :499-506

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