Dietary hydroxy fatty acids are absorbed in humans: Implications for the measurement of 'oxidative stress' in vivo

Lipid peroxidation products formed in vivo or originating from the diet may lead to atherosclerosis. However, little is known about the absorption of these products in man. We studied the absorption of fat (30 g) containing 14-15 mg [(UC)-C-13] -labeled hydroxy or dihydroxy triglycerides in two groups of six apparently healthy women aged 40+/-2 years. Post-prandial C-13-labeled hydroxy fatty acid concentration increased in a pattern somewhat different from that of plasma triglycerides, with peak levels being reached between 4 and 6 h. However, the amount of C-13-labeled oxidized fat absorbed (area under the curve of plasma concentrations from 0 to 8 h) was related to that of plasma triglycerides: C-13 hydroxy vs TG (r=0.88, p<.02), and C-13 dihydroxy vs TG (r=0.85, p<.05). C-13 monohydroxy triglycerides appeared to be absorbed to a greater extent than those of C-13 dihydroxy triglycerides. Although low levels of C-13 hydroxy lipids could be detected in fasting plasma after 24 h, concentrations were very low. Dietary lipid oxidation products are absorbed. The measurement of hydroxy fatty acids in plasma total lipids may not be a valid marker of lipid peroxidation in vivo when subjects are not fasting. (C) 2002 Elsevier Science Inc.