High fractions of exogenous DNA in human buccal samples reduce the quality of large-scale genotyping

被引：14

作者：

Herraez, David Lopez ^{[1
]}

Stoneking, Mark ^{[1
]}

机构：

[1] Max Planck Inst Evolutionary Anthropol, D-04103 Leipzig, Germany

来源：

ANALYTICAL BIOCHEMISTRY | 2008年 / 383卷 / 02期

关键词：

D O I：

10.1016/j.ab.2008.08.015

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Buccal cell samples are considered a reliable source of DNA for genotyping studies. However, a potential drawback is the presence of exogenous DNA that is coextracted with human genomic DNA. A set of saliva and cheek swab samples, in which the fraction of human DNA varies from 10 to 96%, was genotyped using the Affymetrix Mapping 500 K Array. Samples containing less than 30% human DNA performed poorly in terms of accuracy and reliability. Therefore, we recommend quantitating the amount of human DNA in buccal samples to be used for large-scale genotyping and eliminating samples with less than 30% human DNA. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：329 / 331

页数：3

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