The anti-diabetic properties of Guazuma ulmifolia Lam are mediated by the stimulation of glucose uptake in normal and diabetic adipocytes without inducing adipogenesis

被引:58
作者
Alonso-Castro, Angel Josabad [1 ]
Salazar-Olivo, Luis A. [1 ]
机构
[1] Inst Potosino Invest Cient & Tecnol, Div Mol Biol, San Luis Potosi 78216, SLP, Mexico
关键词
Guazuma ulmifolia Lam (Sterculiaceae); 3T3; adipogenesis; 2-NBDG uptake;
D O I
10.1016/j.jep.2008.04.007
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Ethnopharmacological importance: Guazuma ulmifolia Lam (Sterculiaceae) is a plant extensively used in Mexico for the empirical treatment of type 2 diabetes. Aim of the study: To investigate the anti-diabetic mechanisms of Guazuma ulmifolia. Materials and methods: Non-toxic concentrations of Guazuma ulmifolia aqueous extracts (GAE) were assayed on adipogenesis and 2-NBDglucose uptake in the murine 3T3-F442A preadipose cell line. Results: GAE added to adipogenic medium (AM) did not affect adipogenesis at any of the tested concentrations (1-70 mu g/ml), whereas in AM lacking insulin GAE 70 mu g/ml induced triglyceride accumulation by 23%. On the other hand, GAE 70 mu g/ml stimulated 2-NBDG uptake by 40% in insulin-sensitive 3T3-F442A adipocytes and by 24% in insulin-resistant adipocytes, with respect to the incorporation showed by insulin-sensitive adipocytes stimulated with the hormone. Conclusion: Guazuma ulmifolia exerts its anti-diabetic effects by stimulating glucose uptake in both insulin-sensitive and insulin-resistant adipocytes without inducing adipogenesis. (C) 2008 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码:252 / 256
页数:5
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