Mining of camel rumen metagenome to identify novel alkali-thermostable xylanase capable of enhancing the recalcitrant lignocellulosic biomass conversion

被引：37

作者：

Ariaeenejad, Shohreh ^{[1
]}

Maleki, Morteza ^{[1
]}

Hosseini, Elnaz ^{[2
]}

Kavousi, Kaveh ^{[2
]}

Moosavi-Movahedi, Ali A. ^{[2
]}

Salekdeh, Ghasem Hosseini ^{[1
]}

机构：

[1] Agr Res Educ & Extens Org AREO, Dept Syst Biol, ABRII, Karaj, Iran

[2] Univ Tehran, IBB, Tehran, Iran

来源：

BIORESOURCE TECHNOLOGY | 2019年 / 281卷

基金：

美国国家科学基金会;

关键词：

Alkaline thermostable xylanase; Metagenome; Camel rumen; Recalcitrant compounds; BACTERIAL COMMUNITY; IDENTIFICATION; GENE; PURIFICATION; GENOMES; CLONING;

D O I：

10.1016/j.biortech.2019.02.059

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

The aim of this study was to isolate and characterize novel alkali-thermostable xylanase genes from the mixed genome DNA of camel rumen metagenome. In this study, a five-stage computational screening procedure was utilized to find the primary candidate enzyme with superior properties from the camel rumen metagenome. This enzyme was subjected to cloning, purification, and structural and functional characterization. It showed high thermal stability, high activity in a broad range of pH (6-11) and temperature (30-90 degrees C) and effectivity in recalcitrant lignocellulosic biomass degradation. Our results demonstrated the power of in silico analysis to discover novel alkali-thermostable xylanases, effective for the bioconversion of lignocellulosic biomass.

引用

页码：343 / 350

页数：8

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