Transient Expression of Mouse Pro-α3(V) Collagen Gene (Col5a3) in Wound Healing

被引：10
|
作者：
Sumiyoshi, Hideaki [1 ]
Kitamura, Hirokazu [2 ]
Matsuo, Noritaka [1 ]
Tatsukawa, Shuji [2 ]
Ishikawa, Kazushi [3 ]
Okamoto, Osamu [3 ]
Fujikura, Yoshihisa [2 ]
Fujiwara, Sakuhei [3 ]
Yoshioka, Hidekatsu [1 ]
机构：
[1] Oita Univ, Dept Matrix Med, Fac Med, Oita 8795593, Japan
[2] Oita Univ, Dept Mol Anat, Fac Med, Oita 8795593, Japan
[3] Oita Univ, Dept Dermatol, Fac Med, Oita 8795593, Japan
来源：
CONNECTIVE TISSUE RESEARCH | 2012年 / 53卷 / 04期
关键词：
type V collagen; wound healing; gene expression; in situ hybridization; immunoelectron microscopy; EHLERS-DANLOS-SYNDROME; EXPERIMENTAL GRANULATION-TISSUE; V COLLAGEN; I COLLAGEN; CHAIN; ORGANIZATION; EPIPLAKIN; FIBRILS; GROWTH;
D O I：
10.3109/03008207.2011.653061
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
The alpha 3(V) chain is poorly characterized among type V collagen chains. Pro-alpha 3(V) collagen is expressed in newly synthesized bone as well as in the superficial fascia of developing muscle. Present study examined the expression in a mouse model of wound healing. Real-time reverse transcriptase polymerase chain reaction and in situ hybridization revealed transient expression of pro-alpha 3(V) chain at a lower level than other fibrillar collagen genes after injury. Immunohistochemistry showed a similar expression pattern in the injured skin. In addition, electron microscopy showed that pro-alpha 3(V) chain was localized in the amorphous nonfibrillar region, but not in fine or dense fibrils. The pro-alpha 3(V) chain co-localized with heparan sulfate, which appeared in the skin after injury and might bind via an acidic segment of the pro-alpha 3(V) chain. The matrix containing the pro-alpha 3(V) chain may therefore be needed for the initiation of wound healing.
引用
收藏
页码：313 / 317
页数：5
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