Acetylcholinesterase-Catalyzed Hydrolysis Allows Ultrasensitive Detection of Pathogens with the Naked Eye

被引：121

作者：

Liu, Dingbin ^{[1
]}

Wang, Zhantong ^{[2
,3
]}

Jin, Albert ^{[4
]}

Huang, Xinglu ^{[1
]}

Sun, Xiaolian ^{[1
]}

Wang, Fu ^{[1
]}

Yan, Qiang ^{[2
]}

Ge, Shengxiang ^{[2
]}

Xia, Ningshao ^{[2
]}

Niu, Gang ^{[1
]}

Liu, Gang ^{[3
]}

Walker, A. R. Hight ^{[5
]}

Chen, Xiaoyuan ^{[1
]}

机构：

[1] NIBIB, Lab Mol Imaging & Nanomed LOMIN, NIH, Bethesda, MD 20892 USA

[2] Xiamen Univ, Sch Publ Hlth, Natl Inst Diagnost & Vaccine Dev Infect Dis, Xiamen 361102, Peoples R China

[3] Xiamen Univ, Sch Publ Hlth, Ctr Mol Imaging & Translat Med, Xiamen 361005, Peoples R China

[4] NIBIB, Lab Cellular Imaging & Macromol Biophys, NIH, Bethesda, MD 20892 USA

[5] NIST, Opt Technol Div, Phys Lab, Gaithersburg, MD 20899 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2013年 / 52卷 / 52期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

acetylcholinesterase; analytical methods; gold; hydrolysis; nanoparticles; COLORIMETRIC DETECTION; ENTEROVIRUS; 71; GOLD NANOPARTICLES; RAPID DETECTION; DNA; GLUCOSE; POINT; CARE;

D O I：

10.1002/anie.201307952

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Seeing is believing: A rapid diagnostic platform for pathogen detection based on the acetylcholinesterase-catalyzed hydrolysis reaction has been developed. Owing to signal amplification strategies, the sensitivity of this assay is comparable to that of PCR. In addition, the readout of the assay is based on solution color change, which can be easily observed by the naked eye alone. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：14065 / 14069

页数：5

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