Functional characterization of a cellulose binding xylanase from Fusarium oxysporum

被引：6
作者：
Christakopoulos, P
Nerinckx, W
Samyn, B
Kekos, D
Macris, B
VanBeeumen, J
Claeyssens, M
机构：
[1] STATE UNIV GHENT, FAC SCI, DEPT BIOCHEM PHYSIOL & MICROBIOL, B-9000 GHENT, BELGIUM
[2] NATL TECH UNIV ATHENS, DEPT CHEM ENGN, GR-15700 ATHENS, GREECE
来源：
BIOTECHNOLOGY LETTERS | 1996年 / 18卷 / 03期
关键词：
D O I：
10.1007/BF00142957
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
An extracellular endoxylanase from Fusarium oxysporum binds onto crystalline cellulose. A small peptide (similar to 2kDa) could be isolated after partial proteolysis of the native protein. It consists of 18 amino acids, is located in the C-terminal region of the protein and corresponds functionally to a cellulose binding domain (CBD), the first one to be reported in a fungal xylanase. The amino acid sequence of this peptide shows no homology with any known CBD.
引用
收藏
页码：349 / 354
页数：6
相关论文
共 13 条
  • [1] PRODUCTION AND PROPERTIES OF A FACTOR-X CELLULOSE-BINDING DOMAIN FUSION PROTEIN
    ASSOULINE, Z
    SHEN, H
    KILBURN, DG
    WARREN, RAJ
    [J]. PROTEIN ENGINEERING, 1993, 6 (07): : 787 - 792
  • [2] DIRECT FERMENTATION OF CELLULOSE TO ETHANOL BY FUSARIUM-OXYSPORUM
    CHRISTAKOPOULOS, P
    MACRIS, BJ
    KEKOS, D
    [J]. ENZYME AND MICROBIAL TECHNOLOGY, 1989, 11 (04) : 236 - 239
  • [3] CHRISTAKOPOULOS P, 1995, FM LANDBOUWW U GENT, V60, P2005
  • [4] ADSORPTION OF CELLULASES ON STEAM-PRETREATED WILLOW
    GALBE, M
    EKLUND, R
    ZACCHI, G
    [J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 1990, 24-5 : 87 - 101
  • [5] GREENWOOD JM, 1992, PROTEIN ENG, V5, P361
  • [6] STRUCTURE OF THE CATALYTIC CORE OF THE FAMILY F-XYLANASE FROM PSEUDOMONAS-FLUORESCENS AND IDENTIFICATION OF THE XYLOPENTAOSE-BINDING SITES
    HARRIS, GW
    JENKINS, JA
    CONNERTON, I
    CUMMINGS, N
    LOLEGGIO, L
    SCOTT, M
    HAZLEWOOD, GP
    LAURIE, JI
    GILBERT, HJ
    PICKERSGILL, RW
    [J]. STRUCTURE, 1994, 2 (11) : 1107 - 1116
  • [7] A STREPTAVIDIN-CELLULOSE-BINDING DOMAIN FUSION PROTEIN THAT BINDS BIOTINYLATED PROTEINS TO CELLULOSE
    LE, KD
    GILKES, NR
    KILBURN, DG
    MILLER, RC
    SADDLER, JN
    WARREN, RAJ
    [J]. ENZYME AND MICROBIAL TECHNOLOGY, 1994, 16 (06) : 496 - 500
  • [8] LEE SB, 1982, BIOTECHNOL BIOENG, V19, P1091
  • [9] ONG E, 1993, ACS SYM SER, V516, P185
  • [10] ADSORPTION OF TRICHODERMA CELLULASE ON CELLULOSE
    PEITERSEN, N
    MEDEIROS, J
    MANDELS, M
    [J]. BIOTECHNOLOGY AND BIOENGINEERING, 1977, 19 (07) : 1091 - 1094
12