Tissue-engineered endothelial cell layers on surface-modified Ti for inhibiting in vitro platelet adhesion

被引：2

作者：

Wang, Xiupeng ^{[1
]}

He, Fupo ^{[2
,3
]}

Li, Xia ^{[1
]}

Ito, Atsuo ^{[1
]}

Sogo, Yu ^{[1
]}

Maruyama, Osamu ^{[2
]}

Kosaka, Ryo ^{[2
]}

Ye, Jiandong ^{[3
,4
]}

机构：

[1] Natl Inst Adv Ind Sci & Technol, Human Technol Res Inst, Tsukuba, Ibaraki 3058566, Japan

[2] Natl Inst Adv Ind Sci & Technol, Human Technol Res Inst, Tsukuba, Ibaraki 3058564, Japan

[3] S China Univ Technol, Sch Mat Sci & Engn, Guangzhou 510641, Guangdong, Peoples R China

[4] Natl Engn Res Ctr Tissue Restorat & Reconstruct, Guangzhou 510006, Guangdong, Peoples R China

来源：

SCIENCE AND TECHNOLOGY OF ADVANCED MATERIALS | 2013年 / 14卷 / 03期

关键词：

endothelial cell; platelet adhesion; fibroblast growth factor-2; ascorbate; apatite; FIBROBLAST-GROWTH-FACTOR; NITRIC-OXIDE SYNTHASE; APATITE COMPOSITE LAYERS; EXTERNAL FIXATION RODS; DRUG-ELUTING STENTS; PROLIFERATION; FGF-2; ACTIVATION; PREVENTION; EXPRESSION;

D O I：

10.1088/1468-6996/14/3/035002

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

A tissue-engineered endothelial layer was prepared by culturing endothelial cells on a fibroblast growth factor-2 (FGF-2)-L-ascorbic acid phosphate magnesium salt n-hydrate (AsMg)-apatite (Ap) coated titanium plate. The FGF-2-AsMg-Ap coated Ti plate was prepared by immersing a Ti plate in supersaturated calcium phosphate solutions supplemented with FGF-2 and AsMg. The FGF-2-AsMg-Ap layer on the Ti plate accelerated proliferation of human umbilical vein endothelial cells (HUVECs), and showed slightly higher, but not statistically significant, nitric oxide release from HUVECs than on as-prepared Ti. The endothelial layer maintained proper function of the endothelial cells and markedly inhibited in vitro platelet adhesion. The tissue-engineered endothelial layer formed on the FGF-2-AsMg-Ap layer is promising for ameliorating platelet activation and thrombus formation on cardiovascular implants.

引用

页数：8

共 43 条

[1]

Baigent C, 2002, BMJ-BRIT MED J, V324, P71, DOI 10.1136/bmj.324.7329.71

[2] Tissue engineering via local gene delivery: Update and future prospects for enhancing the technology [J].

Bonadio, J .

ADVANCED DRUG DELIVERY REVIEWS, 2000, 44 (2-3) :185-194

[3] Collagen/heparin coating on titanium surface improves the biocompatibility of titanium applied as a blood-contacting biomaterial [J].

Chen, Jialong ;

Chen, Cheng ;

Chen, Zhuoyue ;

Chen, Junying ;

Li, Quanli ;

Huang, Nan .

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 2010, 95A (02) :341-349

[4]

Cines DB, 1998, BLOOD, V91, P3527

[5] BASIC FIBROBLAST GROWTH-FACTOR ENHANCES THE COUPLING OF INTIMAL HYPERPLASIA AND PROLIFERATION OF VASA VASORUM IN INJURED RAT ARTERIES [J].

EDELMAN, ER ;

NUGENT, MA ;

SMITH, LT ;

KARNOVSKY, MJ .

JOURNAL OF CLINICAL INVESTIGATION, 1992, 89 (02) :465-473

[6]

ENGELMANN K, 1995, CORNEA, V14, P62

[7] NITRIC OXIDE-GENERATING VASODILATORS AND 8-BROMO-CYCLIC GUANOSINE-MONOPHOSPHATE INHIBIT MITOGENESIS AND PROLIFERATION OF CULTURED RAT VASCULAR SMOOTH-MUSCLE CELLS [J].

GARG, UC ;

HASSID, A .

JOURNAL OF CLINICAL INVESTIGATION, 1989, 83 (05) :1774-1777

[8] STRUCTURAL CHARACTERIZATION AND BIOLOGICAL FUNCTIONS OF FIBROBLAST GROWTH-FACTOR [J].

GOSPODAROWICZ, D ;

FERRARA, N ;

SCHWEIGERER, L ;

NEUFELD, G .

ENDOCRINE REVIEWS, 1987, 8 (02) :95-114

[9] Ascorbic acid enhances endothelial nitric-oxide synthase activity by increasing intracellular tetrahydrobiopterin [J].

Huang, A ;

Vita, JA ;

Venema, RC ;

Keaney, JF .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (23) :17399-17406

[10] BASIC FIBROBLAST GROWTH-FACTOR INCREASES NITRIC-OXIDE SYNTHASE PRODUCTION IN BOVINE ENDOTHELIAL-CELLS [J].

KOSTYK, SK ;

KOUREMBANAS, S ;

WHEELER, EL ;

MEDEIROS, D ;

MCQUILLAN, LP ;

DAMORE, PA ;

BRAUNHUT, SJ .

AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY, 1995, 269 (05) :H1583-H1589

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