Neonatal Periostin Knockout Mice Are Protected from Hyperoxia-Induced Alveolar Simplication

被引:62
作者
Bozyk, Paul D. [1 ]
Bentley, J. Kelley [2 ]
Popova, Antonia P. [2 ]
Anyanwu, Anuli C. [3 ]
Linn, Marisa D. [2 ]
Goldsmith, Adam M. [2 ]
Pryhuber, Gloria S. [5 ]
Moore, Bethany B. [1 ,4 ]
Hershenson, Marc B. [2 ,3 ]
机构
[1] Univ Michigan, Sch Med, Dept Internal Med, Ann Arbor, MI 48109 USA
[2] Univ Michigan, Sch Med, Dept Pediat & Communicable Dis, Ann Arbor, MI USA
[3] Univ Michigan, Sch Med, Dept Mol & Integrat Physiol, Ann Arbor, MI USA
[4] Univ Michigan, Sch Med, Dept Microbiol & Immunol, Ann Arbor, MI 48109 USA
[5] Univ Rochester, Sch Med & Dent, Dept Pediat, Rochester, NY 14642 USA
来源
PLOS ONE | 2012年 / 7卷 / 02期
关键词
MESENCHYMAL STROMAL CELLS; INDUCED LUNG INJURY; BRONCHOPULMONARY-DYSPLASIA; TRANSFORMING GROWTH-FACTOR-BETA-1; LYSYL OXIDASE; GROWTH-FACTOR; MOUSE LUNG; RESPONSES; EXPRESSION; TGF-BETA-1;
D O I
10.1371/journal.pone.0031336
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
In bronchopulmonary dysplasia (BPD), alveolar septae are thickened with collagen and alpha-smooth muscle actin, transforming growth factor (TGF)-beta-positive myofibroblasts. Periostin, a secreted extracellular matrix protein, is involved in TGF-beta-mediated fibrosis and myofibroblast differentiation. We hypothesized that periostin expression is required for hypoalveolarization and interstitial fibrosis in hyperoxia-exposed neonatal mice, an animal model for this disease. We also examined periostin expression in neonatal lung mesenchymal stromal cells and lung tissue of hyperoxia-exposed neonatal mice and human infants with BPD. Two-to-three day-old wild-type and periostin null mice were exposed to air or 75% oxygen for 14 days. Mesenchymal stromal cells were isolated from tracheal aspirates of premature infants. Hyperoxic exposure of neonatal mice increased alveolar wall periostin expression, particularly in areas of interstitial thickening. Periostin co-localized with alpha-smooth muscle actin, suggesting synthesis by myofibroblasts. A similar pattern was found in lung sections of infants dying of BPD. Unlike wild-type mice, hyperoxia-exposed periostin null mice did not show larger air spaces or alpha-smooth muscle-positive myofibroblasts. Compared to hyperoxia-exposed wild-type mice, hyperoxia-exposed periostin null mice also showed reduced lung mRNA expression of alpha-smooth muscle actin, elastin, CXCL1, CXCL2 and CCL4. TGF-beta treatment increased mesenchymal stromal cell periostin expression, and periostin treatment increased TGF-beta-mediated DNA synthesis and myofibroblast differentiation. We conclude that periostin expression is increased in the lungs of hyperoxia-exposed neonatal mice and infants with BPD, and is required for hyperoxia-induced hypoalveolarization and interstitial fibrosis.
引用
收藏
页数:10
相关论文
共 40 条
[1]  
AMY RWM, 1977, J ANAT, V124, P131
[2]   Disrupted pulmonary vasculature and decreased vascular endothelial growth factor, Flt-1, and TIE-2 in human infants dying with bronchopulmonary dysplasia [J].
Bhatt, AJ ;
Pryhuber, GS ;
Huyck, H ;
Watkins, RH ;
Metlay, LA ;
Maniscalco, WM .
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 2001, 164 (10) :1971-1980
[3]   NF-κB Signaling in Fetal Lung Macrophages Disrupts Airway Morphogenesis [J].
Blackwell, Timothy S. ;
Hipps, Ashley N. ;
Yamamoto, Yasutoshi ;
Han, Wei ;
Barham, Whitney J. ;
Ostrowski, Michael C. ;
Yull, Fiona E. ;
Prince, Lawrence S. .
JOURNAL OF IMMUNOLOGY, 2011, 187 (05) :2740-2747
[4]   Periostin facilitates eosinophil tissue infiltration in allergic lung and esophageal responses [J].
Blanchard, C. ;
Mingler, M. K. ;
McBride, M. ;
Putnam, P. E. ;
Collins, M. H. ;
Chang, G. ;
Stringer, K. ;
Abonia, J. P. ;
Molkentin, J. D. ;
Rothenberg, M. E. .
MUCOSAL IMMUNOLOGY, 2008, 1 (04) :289-296
[5]  
Bozyk PD, 2011, STEM CELLS IN PRESS
[6]  
Burns JS, 2010, TISSUE ENG PT A, V16, P2331, DOI [10.1089/ten.tea.2009.0735, 10.1089/ten.TEA.2009.0735]
[7]  
Coalson Jacqueline J, 2003, Semin Neonatol, V8, P73, DOI 10.1016/S1084-2756(02)00193-8
[8]   Periostin, a member of a novel family of vitamin K-dependent proteins, is expressed by mesenchymal stromal cells [J].
Coutu, Daniel L. ;
Wu, Jian Hui ;
Monette, Anne ;
Rivard, Georges-Etienne ;
Blostein, Mark D. ;
Galipeau, Jacques .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2008, 283 (26) :17991-18001
[9]   Transfer of the active form of transforming growth factor-β1 gene to newborn rat lung induces changes consistent with bronchopulmonary dysplasia [J].
Gauldie, J ;
Galt, T ;
Bonniaud, P ;
Robbins, C ;
Kelly, M ;
Warburton, D .
AMERICAN JOURNAL OF PATHOLOGY, 2003, 163 (06) :2575-2584
[10]   Lung cells from neonates show a mesenchymal stem cell phenotype [J].
Hennrick, Kenneth T. ;
Keeton, Angela G. ;
Nanua, Suparna ;
Kijek, Theresa G. ;
Goldsmith, Adam M. ;
Sajjan, Umadevi S. ;
Bentley, J. Kelley ;
Lama, Vibha N. ;
Moore, Bethany B. ;
Schumacher, Robert E. ;
Thannickal, Victor J. ;
Hershenson, Marc B. .
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 2007, 175 (11) :1158-1164