Magnetic resonance imaging of isolated single liposome by magnetic resonance force microscopy

被引:21
作者
Tsuji, S
Masumizu, T
Yoshinari, Y
机构
[1] JEOL Ltd, Adv Technol Div, Akishima, Tokyo 1968558, Japan
[2] JEOL Ltd, Applicat & Res Ctr, Akishima, Tokyo 1968558, Japan
关键词
MRFM; imaging; ESR; NMR; liposome;
D O I
10.1016/j.jmr.2003.12.011
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Magnetic resonance imaging (MRI) is very useful spectroscopy to visualize a three-dimensional (3D) real structure inside the sample without physical destruction. The spatial resolution of the readily available MRI spectrometer is, however, limited by a few ten to hundreds of microns due to a technological boundary of generating larger magnetic field gradient and to the insensitivity inherent to the inductive signal detection. Magnetic resonance force microscopy (MRFM) is new alternative MRI spectroscopy which is anticipated to significantly surpass the conventional MRI in both resolution and sensitivity. We report two imaging experiments on our MRFM spectrometer operated at room temperature and in vacuum similar to10(-3) pa. One is for similar to20 mum liposome membrane labeled entirely by a nitroxide imaging agent and the other for similar to15 mum DPPH particles, both are nearly the same size as that of human cell. The reconstructed images at spatial resolution similar to1 mum were in satisfactory agreement with the scanning electron microscope images. The potential capability of visualizing intrinsic radicals in the cell is suggested to investigate redox process from a microscopic point of view. (C) 2004 Elsevier Inc. All rights reserved.
引用
收藏
页码:211 / 220
页数:10
相关论文
共 33 条
[1]  
[Anonymous], FREE RADICALS BIOL M
[2]  
[Anonymous], 1974, METHODS MEMBRANE BIO, DOI DOI 10.1002/bit.21710
[3]   Force-detected magnetic resonance in a field gradient of 250 000 Tesla per meter [J].
Bruland, KJ ;
Dougherty, WM ;
Garbini, JL ;
Sidles, JA ;
Chao, SH .
APPLIED PHYSICS LETTERS, 1998, 73 (21) :3159-3161
[4]   ANHARMONIC MODULATION FOR NOISE-REDUCTION IN MAGNETIC-RESONANCE FORCE MICROSCOPY [J].
BRULAND, KJ ;
KRZYSTEK, J ;
GARBINI, JL ;
SIDLES, JA .
REVIEW OF SCIENTIFIC INSTRUMENTS, 1995, 66 (04) :2853-2856
[5]   Magnetic resonance imaging of biological cells [J].
Ciobanu, L ;
Webb, AG ;
Pennington, CH .
PROGRESS IN NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY, 2003, 42 (3-4) :69-93
[6]   3D MR microscopy with resolution 3.7 μm by 3.3 μm by 3.3 μm [J].
Ciobanu, L ;
Seeber, DA ;
Pennington, CH .
JOURNAL OF MAGNETIC RESONANCE, 2002, 158 (1-2) :178-182
[7]   The Bloch equations in high-gradient magnetic resonance force microscopy: Theory and experiment [J].
Dougherty, WM ;
Bruland, KJ ;
Chao, SH ;
Garbini, JL ;
Jensen, SE ;
Sidles, JA .
JOURNAL OF MAGNETIC RESONANCE, 2000, 143 (01) :106-119
[8]   The magnetic-resonance force microscope: A new tool for high-resolution, 3-D, subsurface scanned probe imaging [J].
Hammel, PC ;
Pelekhov, DV ;
Wigen, PE ;
Gosnell, TR ;
Midzor, MM ;
Roukes, ML .
PROCEEDINGS OF THE IEEE, 2003, 91 (05) :789-798
[9]   Nanoscale Fourier-transform imaging with magnetic resonance force microscopy [J].
Kempf, JG ;
Marohn, JA .
PHYSICAL REVIEW LETTERS, 2003, 90 (08) :087601
[10]   Mechanical detection of nuclear spin relaxation in a micron-size crystal [J].
Klein, O ;
Naletov, VV ;
Alloul, H .
EUROPEAN PHYSICAL JOURNAL B, 2000, 17 (01) :57-68