DLK2 regulates arbuscule hyphal branching during arbuscular mycorrhizal symbiosis

被引:24
作者
Ho-Plagaro, Tania [1 ]
Morcillo, Rafael J. L. [2 ]
Tamayo-Navarrete, Maria Isabel [1 ]
Huertas, Raul [3 ]
Molinero-Rosales, Nuria [1 ]
Lopez-Raez, Juan Antonio [1 ]
Macho, Alberto P. [2 ]
Garcia-Garrido, Jose Manuel [1 ]
机构
[1] CSIC, Estn Expt Zaidin EEZ, Dept Soil Microbiol & Symbiot Syst, Calle Prof Albareda 1, Granada 18008, Spain
[2] Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Ctr Plant Stress Biol, CAS Ctr Excellence Mol Plant Sci, Shanghai 201602, Peoples R China
[3] Noble Res Inst LLC, 2510 Sam Noble Pkwy, Ardmore, OK 73401 USA
关键词
arbuscular mycorrhiza; arbuscule formation; butenolide signaling; DLK2; protein; tomato; STRIGOLACTONE BIOSYNTHESIS; TRANSCRIPTION FACTOR; GENE-EXPRESSION; RESPONSES; FUNGI; PERCEPTION; PROTEIN; ROOTS; RICE; INHIBITION;
D O I
10.1111/nph.16938
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
D14 and KAI2 receptors enable plants to distinguish between strigolactones (SLs) and karrikins (KARs), respectively, in order to trigger appropriate environmental and developmental responses. Both receptors are related to the regulation of arbuscular mycorrhiza (AM) formation and are members of the RsbQ-like family of alpha,beta-hydrolases. DLK2 proteins, whose function remains unknown, constitute a third clade from the RsbQ-like protein family. We investigated whether the tomatoSlDLK2is a new regulatory component in the AM symbiosis. Genetic approaches were conducted to analyzeSlDLK2expression and to understand SlDLK2 function in AM symbiosis. We show thatSlDLK2expression in roots is AM-dependent and is associated with cells containing arbuscules.SlDLK2ectopic expression arrests arbuscule branching and downregulates AM-responsive genes, even in the absence of symbiosis; while the opposite effect was observed uponSlDLK2silencing. Moreover,SlDLK2overexpression inMedicago truncatularoots showed the same altered phenotype observed in tomato roots. Interestingly, SlDLK2 interacts with DELLA, a protein that regulates arbuscule formation/degradation in AM roots. We propose that SlDLK2 is a new component of the complex plant-mediated mechanism regulating the life cycle of arbuscules in AM symbiosis.
引用
收藏
页码:548 / 562
页数:15
相关论文
共 68 条
[1]   Plant sesquiterpenes induce hyphal branching in arbuscular mycorrhizal fungi [J].
Akiyama, K ;
Matsuzaki, K ;
Hayashi, H .
NATURE, 2005, 435 (7043) :824-827
[2]   Structural Requirements of Strigolactones for Hyphal Branching in AM Fungi [J].
Akiyama, Kohki ;
Ogasawara, Shin ;
Ito, Seisuke ;
Hayashi, Hideo .
PLANT AND CELL PHYSIOLOGY, 2010, 51 (07) :1104-1117
[3]   Differential expression analysis for sequence count data [J].
Anders, Simon ;
Huber, Wolfgang .
GENOME BIOLOGY, 2010, 11 (10)
[4]   HTSeq-a Python']Python framework to work with high-throughput sequencing data [J].
Anders, Simon ;
Pyl, Paul Theodor ;
Huber, Wolfgang .
BIOINFORMATICS, 2015, 31 (02) :166-169
[5]   Strigolactone regulates shoot development through a core signalling pathway [J].
Bennett, Tom ;
Liang, Yueyang ;
Seale, Madeleine ;
Ward, Sally ;
Mueller, Doerte ;
Leyser, Ottoline .
BIOLOGY OPEN, 2016, 5 (12) :1806-1820
[6]   LIFE-CYCLE OF GLOMUS-INTRARADIX IN ROOT ORGAN-CULTURE [J].
CHABOT, S ;
BECARD, G ;
PICHE, Y .
MYCOLOGIA, 1992, 84 (03) :315-321
[7]   Spatially inhomogeneous competition between superconductivity and the charge density wave in YBa2Cu3O6.67 [J].
Choi, J. ;
Ivashko, O. ;
Blackburn, E. ;
Liang, R. ;
Bonn, D. A. ;
Hardy, W. N. ;
Holmes, A. T. ;
Christensen, N. B. ;
Hucker, M. ;
Gerber, S. ;
Gutowski, O. ;
Ruett, U. ;
Zimmermann, M. v. ;
Forgan, E. M. ;
Hayden, S. M. ;
Chang, J. .
NATURE COMMUNICATIONS, 2020, 11 (01)
[8]   Origin of strigolactones in the green lineage [J].
Delaux, Pierre-Marc ;
Xie, Xiaonan ;
Timme, Ruth E. ;
Puech-Pages, Virginie ;
Dunand, Christophe ;
Lecompte, Emilie ;
Delwiche, Charles F. ;
Yoneyama, Koichi ;
Becard, Guillaume ;
Sejalon-Delmas, Nathalie .
NEW PHYTOLOGIST, 2012, 195 (04) :857-871
[9]   A compound from smoke that promotes seed germination [J].
Flematti, GR ;
Ghisalberti, EL ;
Dixon, KW ;
Trengove, RD .
SCIENCE, 2004, 305 (5686) :977-977
[10]   A Transcriptional Program for Arbuscule Degeneration during AM Symbiosis Is Regulated by MYB1 [J].
Floss, Daniela S. ;
Gomez, S. Karen ;
Park, Hee-Jin ;
MacLean, Allyson M. ;
Mueller, Lena M. ;
Bhattarai, Kishor K. ;
Levesque-Tremblay, Veronique ;
Maldonado-Mendoza, Ignacio E. ;
Harrison, Maria J. .
CURRENT BIOLOGY, 2017, 27 (08) :1206-1212