Retinoic acid 4-hydroxylase-mediated catabolism of all-trans retinoic acid and the cell proliferation in head and neck squamous cell carcinoma

被引:8
作者
Kim, SY
Yoo, SJ
Kwon, HJ
Kim, SH
Byun, Y
Lee, KS
机构
[1] Univ Ulsan, Coll Med, Asan Med Ctr, Dept Otolaryngol, Seoul 138736, South Korea
[2] Sungkyunkwan Univ, Coll Med, Samsung Med Ctr, Dept Clin Pathol, Seoul, South Korea
[3] Kwangju Inst Sci & Technol, Dept Mat Sci & Technol, Kwangju, South Korea
来源
METABOLISM-CLINICAL AND EXPERIMENTAL | 2002年 / 51卷 / 04期
关键词
D O I
10.1053/meta.2002.31335
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
All-trans retinoic acid (RA) can be catabolized to polar metabolites by microsomal P450s (P450). The aim of this study was to confirm if retinoic acid 4-hydroxylase (CYP26) is a P450 induced by RA and to investigate the role of cellular RA binding proteins (CRABPs), using a slow catabolizer, AMC-HN-4, and a rapid catabolizer, AMC-HN-6. Also, we analyzed the effect of RA catabolism on cell proliferation of head and neck squamous cell carcinoma (HNSCC) in vitro and in vivo. Both cell lines weakly expressed CYP26 and CRABPs, but RA induced CYP26 only in AMC-HN-6. The sensitivity to RA was variable by the amount of CYP26, and the rapid catabolism by CYP26 made AMC-HN-6 resistant to RA in vitro. In addition, The RA had a stronger effect on the inhibition of tumor growth of AMC-HN-4 than that of AMC-HN-6 in vivo. Conclusively, the CYP26 activity might be one essential factor for the RA sensitivity, but in cells showing induction of CYP26, the RA sensitivity is inversely related to the rate of RA catabolism. Copyright 2002, Elsevier Science (USA). All rights reserved.
引用
收藏
页码:477 / 481
页数:5
相关论文
共 20 条
  • [1] BENNER SE, 1994, SEMIN HEMATOL, V31, P26
  • [2] BOYLAN JF, 1992, J BIOL CHEM, V267, P21486
  • [3] DETERMINATION OF ISOTRETINOIN OR ETRETINATE AND THEIR MAJOR METABOLITES IN HUMAN-BLOOD BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
    BUGGE, CJL
    RODRIGUEZ, LC
    VANE, FM
    [J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 1985, 3 (03) : 269 - 277
  • [4] EXPRESSION OF CELLULAR RETINOID-BINDING PROTEINS DURING NORMAL AND ABNORMAL EPIDERMAL DIFFERENTIATION
    BUSCH, C
    SIEGENTHALER, G
    VAHLQUIST, A
    NORDLINDER, H
    SUNDELIN, J
    SAKSENA, P
    ERIKSSON, U
    [J]. JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1992, 99 (06) : 795 - 802
  • [5] THE MOLECULAR-CLONING AND EXPRESSION OF 2 CRABP CDNAS FROM HUMAN SKIN
    ELLER, MS
    OLEKSIAK, MF
    MCQUAID, TJ
    MCAFEE, SG
    GILCHREST, BA
    [J]. EXPERIMENTAL CELL RESEARCH, 1992, 198 (02) : 328 - 336
  • [6] Giannini F, 1997, INT J CANCER, V70, P194
  • [7] Hong Waun Ki, 1994, P597
  • [8] Isogai M, 1997, CANCER RES, V57, P4460
  • [9] Establishment and characterization of nine new head and neck cancer cell lines
    Kim, SY
    Chu, KC
    Lee, HR
    Lee, KS
    Carey, TE
    [J]. ACTA OTO-LARYNGOLOGICA, 1997, 117 (05) : 775 - 784
  • [10] The induction of P450-mediated oxidation of all-trans retinoic acid by retinoids in head and neck squamous cell carcinoma cell lines
    Kim, SY
    Han, IS
    Yu, HK
    Lee, HR
    Chung, JW
    Choi, JH
    Kim, SH
    Byun, Y
    Carey, TE
    Lee, KS
    [J]. METABOLISM-CLINICAL AND EXPERIMENTAL, 1998, 47 (08): : 955 - 958