The effect of PEG-5K grafting level and particle size on tumor accumulation and cellular uptake

被引：24

作者：

Lo, Chun-Liang ^{[1
,2
]}

Chou, Meng-Han ^{[3
]}

Lu, Pei-Lin ^{[4
,5
]}

Lo, I-Wen ^{[1
]}

Chiang, Yi-Ting ^{[1
]}

Hung, Shang-Yu ^{[1
]}

Yang, Chieh-Yu ^{[1
]}

Lin, Shuian-Yin ^{[6
]}

Wey, Shiaw-Pyng ^{[4
,5
]}

Lo, Jem-Mau ^{[3
]}

Hsiue, Ging-Ho ^{[7
]}

机构：

[1] Natl Yang Ming Univ, Dept Biomed Engn, Taipei 112, Taiwan

[2] Natl Yang Ming Univ, BMIRC, Taipei 112, Taiwan

[3] Natl Tsing Hua Univ, Dept Biomed Engn & Environm Sci, Hsinchu 300, Taiwan

[4] Chang Gung Mem Hosp, Dept Nucl Med, Tao Yuan 333, Taiwan

[5] Chang Gung Mem Hosp, Mol Imaging Ctr, Tao Yuan 333, Taiwan

[6] Natl Tsing Hua Univ, Dept Chem Engn, Hsinchu 300, Taiwan

[7] Chung Yuan Christian Univ, R&D Ctr Membrane Technol, Chungli 320, Taiwan

来源：

INTERNATIONAL JOURNAL OF PHARMACEUTICS | 2013年 / 456卷 / 02期

关键词：

Gold nanoparticles; Surface modification; Polyethylene glycol; Cellular uptake; Biodistribution; COATED GOLD NANOPARTICLES; IN-VIVO BIODISTRIBUTION; DRUG-DELIVERY; CANCER-CHEMOTHERAPY; PLASMA-PROTEINS; HEPATIC-UPTAKE; ENDOCYTOSIS; ASSEMBLIES; INJECTION; MECHANISM;

D O I：

10.1016/j.ijpharm.2013.08.045

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

PEG-modified gold nanoparticles (PEG-modified GNs) with diameters of 40 nm and 70 nm were prepared to elucidate the effect of extent of PEG (M.W. 5000) grafting and particle size on tumor accumulation and cellular uptake. Flow cytometry reveals that cellular uptake is strongly related to the size of PEG-modified GNs, rather than the extent of PEG-5K grafting level. Cytotoxicity analysis based on the intracellular release of drugs showed that the 70 nm PEG-modified GNs have the higher cytotoxicity, beccause of their greater cellular uptake. Also, particle size, rather than PEG-5K grafting level affects tumor accumulation. However, PEG-5K grafting level significantly affects the accumulation of particles in the liver and spleen. This finding is important in determining the proper PEG-5K grafting level and particle size for designing nano-medicines. (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：424 / 431

页数：8

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