共 45 条
Macroautophagy Proteins Assist Epstein Barr Virus Production and Get Incorporated Into the Virus Particles
被引:84
作者:
Nowag, Heike
[1
]
Guhl, Bruno
[2
]
Thriene, Kerstin
[3
,4
,5
,6
]
Romao, Susana
[1
]
Ziegler, Urs
[2
]
Dengjel, Joern
[3
,4
,5
,6
]
Munz, Christian
[1
]
机构:
[1] Univ Zurich, Inst Expt Immunol, Viral Immunobiol, CH-8057 Zurich, Switzerland
[2] Univ Zurich, Ctr Microscopy & Image Anal, CH-8057 Zurich, Switzerland
[3] Univ Freiburg, Med Ctr, Dept Dermatol, D-79104 Freiburg, Germany
[4] Univ Freiburg, Freiburg Inst Adv Studies, D-79104 Freiburg, Germany
[5] Univ Freiburg, BIOSS Ctr Biol Signalling Studies, D-79104 Freiburg, Germany
[6] Univ Freiburg, ZBSA Ctr Biol Syst Anal, D-79104 Freiburg, Germany
基金:
英国医学研究理事会;
英国惠康基金;
瑞士国家科学基金会;
关键词:
Atg8/LC3;
Atg12;
Atg16;
BZLF1;
Lytic EBV replication;
Epithelial cell;
B cell;
AUTOPHAGY MACHINERY;
NUCLEAR ANTIGEN;
CELLS;
INFECTION;
COMPARTMENTS;
BIOGENESIS;
LIPIDATION;
MOLECULES;
RESPONSES;
LESSONS;
D O I:
10.1016/j.ebiom.2014.11.007
中图分类号:
R5 [内科学];
学科分类号:
1002 ;
100201 ;
摘要:
Epstein Barr virus (EBV) persists as a latent herpes virus infection in the majority of the adult human population. The virus can reactivate from this latent infection into lytic replication for virus particle production. Here, we report that autophagic membranes, which engulf cytoplasmic constituents during macroautophagy and transport them to lysosomal degradation, are stabilized by lytic EBV replication in infected epithelial and B cells. Inhibition of autophagic membrane formation compromises infectious particle production and leads to the accumulation of viral DNA in the cytosol. Vice versa, pharmacological stimulation of autophagic membrane formation enhances infectious virus production. Atg8/LC3, an essential macroautophagy protein and substrate anchor on autophagic membranes, was found in virus preparations, suggesting that EBV recruits Atg8/LC3 coupled membranes to its envelope in the cytosol. Our data indicate that EBV subverts macroautophagy and uses autophagic membranes for efficient envelope acquisition during lytic infection. (C) 2014 The Authors. Published by Elsevier B.V.
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页码:116 / 125
页数:10
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