Validation of a confirmatory method for the determination of sulphonamides in muscle according to the European Union regulation 2002/657/EC

被引：69

作者：

Pecorelli, I ^{[1
]}

Bibi, R ^{[1
]}

Fioroni, L ^{[1
]}

Galarini, R ^{[1
]}

机构：

[1] Ist Zooprofilatt Sperimentale Umbria & Marche, I-06126 Perugia, Italy

来源：

JOURNAL OF CHROMATOGRAPHY A | 2004年 / 1032卷 / 1-2期

关键词：

validation; sulphonamides;

D O I：

10.1016/j.chroma.2003.11.010

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple multiresidue method is described for assaying 10 sulphonamides (SAs) (sulfadiazine, sulfathiazole, sulfapyridine, sulfamerazine, sulfamethazine, sulfamonomethoxine, sulfachlorpyridazine, sulfamethoxazole, sulfaquinoxaline and sulfadimethoxine) in muscle samples. Samples were prepared by homogenizing the tissue, extracting with ethyl acetate and cleaning up with a cation-exchange solid-phase extraction (SPE) column. The detection of analytes was achieved by HPLC-diode array detection (DAD) at 270 nm. The procedure was validated according to the European Union regulation 2002/657/EC determining specificity, decision limit, detection capability, trueness and precision. The results of validation process demonstrate that the method is suitable for application in European Union statutory veterinary drug residue surveillance programmes. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：23 / 29

页数：7

共 21 条

[1]

AKKUNDI S, 2003, J CHROMATOGR B, V788, P29

[2]

[Anonymous], 1997, 118431 ISO

[3]

[Anonymous], 1988, FED REG

[4]

[Anonymous], 1999, OFF J EUR COMMUN L, VL128

[5]

[Anonymous], 2002, OFF J EUR COMMUNITIE

[6] Validation of analytical methods based on mass spectrometric detection according to the "2002/657/EC" European decision: guideline and application [J].

Antignac, JP ;

Le Bizec, B ;

Monteau, F ;

Andre, F .

ANALYTICA CHIMICA ACTA, 2003, 483 (1-2) :325-334

[7] Determination of enrofloxacin and its metabolite ciprofloxacin in goat milk by high-performance liquid chromatography with diode-array detection - Optimization and validation [J].

Cinquina, AL ;

Roberti, P ;

Giannetti, L ;

Longo, F ;

Draisci, R ;

Fagiolo, A ;

Brizioli, NR .

JOURNAL OF CHROMATOGRAPHY A, 2003, 987 (1-2) :221-226

[8] Quantitative determination of sulfonamide in meat by liquid chromatography-electrospray-mass spectrometry [J].

Fuh, MRS ;

Chan, SA .

TALANTA, 2001, 55 (06) :1127-1139

[9]

Gehring TA, 1997, J AOAC INT, V80, P751

[10]

Gehring TA, 1995, J AOAC INT, V78, P1161

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