RAT MODEL OF BURN WOUND HEALING: EFFECT OF BOTOX

被引：1
作者：
Hussein, I. Abdallah Hajj [2 ]
Balta, N. Dali [2 ]
Jurjus, R. A. [3 ]
Watfa, W. [4 ]
Gerges, A. [4 ]
Atiyeh, B. [5 ]
Pullar, C.
Leone, A. [1 ,6 ]
Jurjus, A. R. [2 ]
机构：
[1] Univ Palermo, Sect Histol & Embryol, Dept Expt Biomed & Clin Neurosci, I-90129 Palermo, Italy
[2] Amer Univ Beirut, Dept Anat Cell Biol & Physiol, Beirut, Lebanon
[3] George Washington Univ, Dept Anat & Regenerat Biol, Washington, DC USA
[4] Lebanese Hlth Soc, Beirut, Lebanon
[5] Amer Univ Beirut, Dept Surg, Beirut, Lebanon
[6] Univ Leicester, Dept Cell Physiol & Pharmacol, Leicester LE1 7RH, Leics, England
来源：
JOURNAL OF BIOLOGICAL REGULATORS AND HOMEOSTATIC AGENTS | 2012年 / 26卷 / 03期
关键词：
botox; burn; animal models; TGF-beta; TNF-alpha; TOXIN TYPE-A; BOTULINUM-TOXIN; GROWTH-FACTORS; MODULATION;
D O I：
暂无
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
Animal models of burn play a crucial role in studying the mechanisms of burn wound progression and the factors that regulate various stages of healing. In this study, using a rat model, we assessed the effect of Botox in the healing process through parameters like transepidermal water loss (TEWL), histological alterations, transforming growth factor beta (TGF-beta(1)) and tumor necrosis factor alpha (TNF-alpha). Fifty Sprague-Dawley rats were inflicted with 5 cm(2) second degree burn and divided into 2 groups; one group was injected intralesionally with Botox and the other with saline. Daily observation and transepidermal water loss measurement were performed. Biopsies were taken on days 0, 3, 8, 14, and 28 for histology and polymerase chain reaction, testing TGF-beta and TNF-alpha. The results showed no significant difference in TEWL except for slightly better preservation of moisture with Botox. Histology revealed relatively better and faster regeneration with Botox, delayed lower grade inflammation, and increase in fibroblasts. TNF-alpha had an acute increase of 21-fold then tapered down while TGF-beta levels increased on day 3 after TNF-alpha, peaked on day 8 and then started to decrease until complete healing. Botox improved the healing process and the cosmetic appearance of burn scar.
引用
收藏
页码：389 / 400
页数：12
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