Implications of macromolecular crowding for protein-protein association kinetics in the cytoplasm of living cells

被引：18

作者：

Tabaka, Marcin ^{[1
]}

Sun, Lili ^{[1
]}

Kalwarczyk, Tomasz ^{[1
]}

Holyst, Robert ^{[1
]}

机构：

[1] Polish Acad Sci, Inst Phys Chem, Warsaw, Poland

来源：

SOFT MATTER | 2013年 / 9卷 / 17期

关键词：

POISSON-BOLTZMANN EQUATION; ESCHERICHIA-COLI; ROTATIONAL DIFFUSION; VISCOSITY; DYNAMICS; FIELD;

D O I：

10.1039/c3sm00013c

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

We present a method of prediction of in vivo association rate constants between proteins from in vitro rate constants. This model accounts for the changes in length-scale dependent viscosity of the cytoplasm of a living cell and interaction potential between proteins. We explain the unexpected results obtained for protein-protein association rate constants in the cytoplasm of HeLa cells determined by Phillip et al. Proc. Natl. Acad. Sci. U. S. A., 2012, 109, 1461.

引用

收藏

页码：4386 / 4389

页数：4

相关论文

共 29 条

[1] Temporal Analysis of Active and Passive Transport in Living Cells [J].

Arcizet, Delphine ;

Meier, Boern ;

Sackmann, Erich ;

Raedler, Joachim O. ;

Heinrich, Doris .

PHYSICAL REVIEW LETTERS, 2008, 101 (24)

[2] Physical limits of cells and proteomes [J].

Dill, Ken A. ;

Ghosh, Kingshuk ;

Schmit, Jeremy D. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2011, 108 (44) :17876-17882

[3] Macromolecular crowding: obvious but underappreciated [J].

Ellis, RJ .

TRENDS IN BIOCHEMICAL SCIENCES, 2001, 26 (10) :597-604

[4] Macromolecular crowding: an important but neglected aspect of the intracellular environment [J].

Ellis, RJ .

CURRENT OPINION IN STRUCTURAL BIOLOGY, 2001, 11 (01) :114-119

[5] HOW CROWDED IS THE CYTOPLASM [J].

FULTON, AB .

CELL, 1982, 30 (02) :345-347

[6] Scaling form of viscosity at all length-scales in poly(ethylene glycol) solutions studied by fluorescence correlation spectroscopy and capillary electrophoresis [J].

Holyst, Robert ;

Bielejewska, Anna ;

Szymanski, Jedrzej ;

Wilk, Agnieszka ;

Patkowski, Adam ;

Gapinski, Jacek ;

Zywocinski, Andrzej ;

Kalwarczyk, Tomasz ;

Kalwarczyk, Ewelina ;

Tabaka, Marcin ;

Ziebacz, Natalia ;

Wieczorek, Stefan A. .

PHYSICAL CHEMISTRY CHEMICAL PHYSICS, 2009, 11 (40) :9025-9032

[7] Biologistics-Diffusion coefficients for complete proteome of Escherichia coli [J].

Kalwarczyk, Tomasz ;

Tabaka, Marcin ;

Holyst, Robert .

BIOINFORMATICS, 2012, 28 (22) :2971-2978

[8] Comparative Analysis of Viscosity of Complex Liquids and Cytoplasm of Mammalian Cells at the Nanoscale [J].

Kalwarczyk, Tomasz ;

Ziebacz, Natalia ;

Bielejewska, Anna ;

Zaboklicka, Ewa ;

Koynov, Kaloian ;

Szymanski, Jedrzej ;

Wilk, Agnieszka ;

Patkowski, Adam ;

Gapinski, Jacek ;

Butt, Hans-Juergen ;

Holyst, Robert .

NANO LETTERS, 2011, 11 (05) :2157-2163

[9] THE NA+,K+,2CL--COTRANSPORT SYSTEM IN HELA-CELLS AND HELA-CELL MUTANTS EXHIBITING AN ALTERED EFFLUX PATHWAY [J].

KORT, JJ ;

KOCH, G .

JOURNAL OF CELLULAR PHYSIOLOGY, 1989, 141 (01) :181-190

[10] Separating the contribution of translational and rotational diffusion to protein association [J].

Kuttner, YY ;

Kozer, N ;

Segal, E ;

Schreiber, G ;

Haran, G .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2005, 127 (43) :15138-15144