Association of murine leukemia virus Pol with virions, independent of Gag-Pol expression

被引：15
作者：
Buchschacher, GL
Yu, L
Murai, F
Friedmann, T
Miyanohara, A
机构：
[1] Univ Calif San Diego, Ctr Mol Genet, Dept Pediat, La Jolla, CA 92093 USA
[2] Univ Calif San Diego, Ctr Mol Genet, Dept Med, Div Hematol Oncol, La Jolla, CA 92093 USA
来源：
JOURNAL OF VIROLOGY | 1999年 / 73卷 / 11期
关键词：
D O I：
10.1128/JVI.73.11.9632-9637.1999
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
During the replication cycle of murine leukemia virus (MLV), Pol is normally synthesized as part of a Gag-Pol fusion protein. In this study, the ability of free MLV Pol to be incorporated into virions was examined. When MLV Gag and MLV Pol were coexpressed from separate plasmids in cells, reverse transcriptase (RT) activity associated with Gag core particles at a slightly lower level than did RT activity generated from wild-type Gag-Pol expression. Particles produced in this manner were somewhat less infectious than those produced with wild-type Gag-Pol. A smaller amount of MLV Pol also associated with heterologous human immunodeficiency virus type 1 Gag cores.
引用
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页码：9632 / 9637
页数：6
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