Stop-flow comprehensive two-dimensional liquid chromatography combined with mass spectrometric detection for phospholipid analysis

被引:64
|
作者
Dugo, Paola [1 ,2 ]
Fawzy, Nermeen [1 ]
Cichello, Filomena [1 ]
Cacciola, Francesco [1 ,3 ]
Donato, Paola [1 ,2 ]
Mondello, Luigi [1 ,2 ]
机构
[1] Univ Messina, Dipartimento Sci Farm & Prod Salute, I-98168 Messina, Italy
[2] Univ Campus Biomed, CIR, I-00128 Rome, Italy
[3] Univ Messina, Dipartimento Sci Farm & Prod Salute, Chromaleont Srl, I-98168 Messina, Italy
关键词
Comprehensive LC; Phospholipids; Mass spectrometry; Stop-flow; EVAPORATIVE LIGHT-SCATTERING; HYDROPHILIC INTERACTION; SEPARATION; PEPTIDES; PHOSPHATIDYLCHOLINE; IDENTIFICATION; PLASMA;
D O I
10.1016/j.chroma.2012.12.042
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A novel comprehensive two-dimensional liquid chromatographic (LC x LC) system for characterization of phospholipid (PL) molecular species belonging to six phospholipid classes was developed. To tackle such a task, a silica hydrophilic interaction liquid chromatography (HILIC) column was used as the first dimension (D-1), and reversed-phase liquid chromatography (RP-LC) with a C18 column was used as the second dimension (D-2) in combination with mass spectrometric detection. Fraction transfer from the D-1 to the D-2 was performed by means of a two-position ten-port switching valve, operated under stop-flow conditions. The capability of the investigated LC x LC approach was demonstrated in the separation of phospholipid molecular species contained in two Folch-extracted cow's milk and plasma samples. Within the phosphatidylcholine class, up to 16 and 14 different species were identified, respectively, by means of the HILIC x RP-LC-MS system employed under both positive and negative ionization mode. (c) 2012 Elsevier B.V. All rights reserved.
引用
收藏
页码:46 / 53
页数:8
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