Stop-flow comprehensive two-dimensional liquid chromatography combined with mass spectrometric detection for phospholipid analysis

被引：65
作者：
Dugo, Paola [1 ,2 ]
Fawzy, Nermeen [1 ]
Cichello, Filomena [1 ]
Cacciola, Francesco [1 ,3 ]
Donato, Paola [1 ,2 ]
Mondello, Luigi [1 ,2 ]
机构：
[1] Univ Messina, Dipartimento Sci Farm & Prod Salute, I-98168 Messina, Italy
[2] Univ Campus Biomed, CIR, I-00128 Rome, Italy
[3] Univ Messina, Dipartimento Sci Farm & Prod Salute, Chromaleont Srl, I-98168 Messina, Italy
来源：
JOURNAL OF CHROMATOGRAPHY A | 2013年 / 1278卷
关键词：
Comprehensive LC; Phospholipids; Mass spectrometry; Stop-flow; EVAPORATIVE LIGHT-SCATTERING; HYDROPHILIC INTERACTION; SEPARATION; PEPTIDES; PHOSPHATIDYLCHOLINE; IDENTIFICATION; PLASMA;
D O I：
10.1016/j.chroma.2012.12.042
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A novel comprehensive two-dimensional liquid chromatographic (LC x LC) system for characterization of phospholipid (PL) molecular species belonging to six phospholipid classes was developed. To tackle such a task, a silica hydrophilic interaction liquid chromatography (HILIC) column was used as the first dimension (D-1), and reversed-phase liquid chromatography (RP-LC) with a C18 column was used as the second dimension (D-2) in combination with mass spectrometric detection. Fraction transfer from the D-1 to the D-2 was performed by means of a two-position ten-port switching valve, operated under stop-flow conditions. The capability of the investigated LC x LC approach was demonstrated in the separation of phospholipid molecular species contained in two Folch-extracted cow's milk and plasma samples. Within the phosphatidylcholine class, up to 16 and 14 different species were identified, respectively, by means of the HILIC x RP-LC-MS system employed under both positive and negative ionization mode. (c) 2012 Elsevier B.V. All rights reserved.
引用
收藏
页码：46 / 53
页数：8
相关论文
共 46 条
[1]  
[Anonymous], HDB DAIRY FOODS ANAL
[2]   A theoretical basis for parameter selection and instrument design in comprehensive size-exclusion chromatography x liquid chromatography [J].
Bedani, Filippo ;
Kok, Wim Th. ;
Janssen, Hans-Gerd .
JOURNAL OF CHROMATOGRAPHY A, 2006, 1133 (1-2) :126-134
[3]   Comprehensive two-dimensional liquid chromatographic analysis of rooibos (Aspalathus linearis) phenolics [J].
Beelders, Theresa ;
Kalili, Kathithileni M. ;
Joubert, Elizabeth ;
de Beer, Dalene ;
de Villiers, Andre .
JOURNAL OF SEPARATION SCIENCE, 2012, 35 (14) :1808-1820
[4]   Two-dimensional and serial column reversed-phase separation of phenolic antioxidants on octadecyl-, polyethyleneglycol-, and pentafluorophenylpropyl-silica columns [J].
Blahová, E ;
Jandera, P ;
Cacciola, F ;
Mondello, L .
JOURNAL OF SEPARATION SCIENCE, 2006, 29 (04) :555-566
[5]   Determination of phospholipid molecular species in pork meat by high performance liquid chromatography-tandem mass spectrometry and evaporative light scattering detection [J].
Boselli, E. ;
Pacetti, D. ;
Curzi, F. ;
Frega, N. G. .
MEAT SCIENCE, 2008, 78 (03) :305-313
[6]   Characterization of Phospholipid Molecular Species in the Edible Parts of Bony Fish and Shellfish [J].
Boselli, Emanuele ;
Pacetti, Deborah ;
Lucci, Paolo ;
Frega, Natale G. .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2012, 60 (12) :3234-3245
[7]  
Danthine S, 2000, LAIT, V80, P209, DOI 10.1051/lait:2000120
[8]   Mass spectrometry detection in comprehensive liquid chromatography: Basic concepts, instrumental aspects, applications and trends [J].
Donato, Paola ;
Cacciola, Francesco ;
Tranchida, Peter Quinto ;
Dugo, Paola ;
Mondello, Luigi .
MASS SPECTROMETRY REVIEWS, 2012, 31 (05) :523-559
[9]   Determination of phospholipids in milk samples by means of hydrophilic interaction liquid chromatography coupled to evaporative light scattering and mass spectrometry detection [J].
Donato, Paola ;
Cacciola, Francesco ;
Cichello, Filomena ;
Russo, Marina ;
Dugo, Paola ;
Mondello, Luigi .
JOURNAL OF CHROMATOGRAPHY A, 2011, 1218 (37) :6476-6482
[10]   Online Comprehensive RPLC x RPLC with Mass Spectrometry Detection for the Analysis of Proteome Samples [J].
Donato, Paola ;
Cacciola, Francesco ;
Sommella, Eduardo ;
Fanali, Chiara ;
Dugo, Laura ;
Dacha, Marina ;
Campiglia, Pietro ;
Novellino, Ettore ;
Dugo, Paola ;
Mondello, Luigi .
ANALYTICAL CHEMISTRY, 2011, 83 (07) :2485-2491
12345