Determining the gross biochemical composition of cells and tissue with Raman spectroscopy

被引：0

作者：

Mourant, Judith R. ^{[1
]}

Dominguez, Jorge ^{[1
]}

Carpenter, Susan ^{[1
]}

Powers, Tamara M. ^{[1
]}

Guerra, Anabel ^{[1
]}

Short, Kurt W. ^{[1
]}

Kunapareddy, Nagapratima ^{[1
]}

Freyer, James P. ^{[1
]}

机构：

[1] Los Alamos Natl Lab, Biosci Div, MS E535, Los Alamos, NM 87545 USA

来源：

BIOMEDICAL VIBRATIONAL SPECTROSCOPY III: ADVANCES IN RESEARCH AND INDUSTRY | 2006年 / 6093卷

关键词：

Raman spectroscopy; biochemical composition of cells; cancer detection; tissue diagnostics;

D O I：

10.1117/12.646809

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

The biochemical composition of mammalian cells has been estimated by Raman spectroscopy and the results compared with other biochemical methods. The Raman spectroscopy estimates were performed by fitting measured Raman and infrared spectra of dense cell suspensions to a linear combination of basis components (RNA, DNA, protein, lipid, glycoen). The Raman spectroscopy results are compared to biochemical analyses performed by extraction and quantification of the biochemical components. Both absolute and relative measurements of biochemical composition are compared. Both the Raman and biochemical results indicate that there are significant differences in gross biochemical composition dependent on growth stage and tumorigneicity.

引用

页数：9

共 50 条

[31] Raman Spectroscopy Analysis of the Biochemical Characteristics of Experimental Keratomycosis [J].

Hu, Jianzhang ;

Wu, Fujin ;

Huang, Zufang ;

Ma, Shuting ;

Zhang, Jingjin ;

Yang, Juan ;

Han, Xiaoli ;

Xu, Guoxing .

CURRENT EYE RESEARCH, 2016, 41 (11) :1408-1413

[32] Raman spectroscopy: elucidation of biochemical changes in carcinogenesis of oesophagus [J].

Shetty, G. ;

Kendall, C. ;

Shepherd, N. ;

Stone, N. ;

Barr, H. .

BRITISH JOURNAL OF CANCER, 2006, 94 (10) :1460-1464

[33] Raman spectroscopy: elucidation of biochemical changes in carcinogenesis of oesophagus [J].

G Shetty ;

C Kendall ;

N Shepherd ;

N Stone ;

H Barr .

British Journal of Cancer, 2006, 94 :1460-1464

[34] Raman spectroscopy:: A powerful technique for biochemical analysis and diagnosis [J].

Moreira, Leonardo M. ;

Silveira, Landulfo, Jr. ;

Santos, Fabio V. ;

Lyon, Juliana P. ;

Rocha, Rick ;

Zangaro, Renato A. ;

Villaverde, Antonio Balbin ;

Pacheco, Marcos T. T. .

SPECTROSCOPY-AN INTERNATIONAL JOURNAL, 2008, 22 (01) :1-19

[35] Analysis of scorpion venom composition by Raman Spectroscopy [J].

Martinez-Zerega, Brenda E. ;

Gonzalez-Solis, Jose L. .

OPTICS AND MEASUREMENT CONFERENCE 2014, 2015, 9442

[36] Determination of Gasoline Composition Based on Raman Spectroscopy [J].

Zhang Bing ;

Deng Zhi-yin ;

Zheng Jing-kui ;

Wang Xiao-ping .

SPECTROSCOPY AND SPECTRAL ANALYSIS, 2015, 35 (06) :1577-1581

[37] Application of Raman Spectroscopy for Determination of Syngas Composition [J].

Petrov, Dmitry V. ;

Matrosov, Ivan I. ;

Zaripov, Alexey R. ;

Maznoy, Anatoly S. .

APPLIED SPECTROSCOPY, 2020, 74 (08) :948-953

[38] Mirrored stainless steel substrate provides improved signal for Raman spectroscopy of tissue and cells [J].

Lewis, Aaran T. ;

Gaifulina, Riana ;

Isabelle, Martin ;

Dorney, Jennifer ;

Woods, Mae L. ;

Lloyd, Gavin R. ;

Lau, Katherine ;

Rodriguez-Justo, Manuel ;

Kendall, Catherine ;

Stone, Nicholas ;

Thomas, Geraint M. .

JOURNAL OF RAMAN SPECTROSCOPY, 2017, 48 (01) :119-125

[39] A Raman spectroscopy bio-sensor for tissue discrimination in surgical robotics [J].

Ashok, Praveen C. ;

Giardini, Mario E. ;

Dholakia, Kishan ;

Sibbett, Wilson .

JOURNAL OF BIOPHOTONICS, 2014, 7 (1-2) :103-109

[40] RAMAN SPECTROSCOPY STUDY OF THE BONE TISSUE WITH COXARTHROSIS [J].

Parkhomenko, Irina N. ;

Vlasukova, Liudmila A. ;

Komarov, Fadey E. ;

Kamyshan, Alexander S. ;

Uryev, Gennadiy A. ;

Murzich, Alexander E. ;

Nemkaeva, Renata R. .

DOKLADY NATSIONALNOI AKADEMII NAUK BELARUSI, 2018, 62 (01) :24-32

← 1 2 3 4 5 →