Determining the gross biochemical composition of cells and tissue with Raman spectroscopy

被引：0
|
作者：
Mourant, Judith R. [1 ]
Dominguez, Jorge [1 ]
Carpenter, Susan [1 ]
Powers, Tamara M. [1 ]
Guerra, Anabel [1 ]
Short, Kurt W. [1 ]
Kunapareddy, Nagapratima [1 ]
Freyer, James P. [1 ]
机构：
[1] Los Alamos Natl Lab, Biosci Div, MS E535, Los Alamos, NM 87545 USA
来源：
BIOMEDICAL VIBRATIONAL SPECTROSCOPY III: ADVANCES IN RESEARCH AND INDUSTRY | 2006年 / 6093卷
关键词：
Raman spectroscopy; biochemical composition of cells; cancer detection; tissue diagnostics;
D O I：
10.1117/12.646809
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
The biochemical composition of mammalian cells has been estimated by Raman spectroscopy and the results compared with other biochemical methods. The Raman spectroscopy estimates were performed by fitting measured Raman and infrared spectra of dense cell suspensions to a linear combination of basis components (RNA, DNA, protein, lipid, glycoen). The Raman spectroscopy results are compared to biochemical analyses performed by extraction and quantification of the biochemical components. Both absolute and relative measurements of biochemical composition are compared. Both the Raman and biochemical results indicate that there are significant differences in gross biochemical composition dependent on growth stage and tumorigneicity.
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页数：9
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