Structural modification of NADPH oxidase activator (Noxa 1) by oxidative stress: An experimental and computational study

被引：22

作者：

Attri, Pankaj ^{[1
,2
]}

Park, Jae-Hyun ^{[3
]}

De Backer, Joey ^{[4
]}

Kim, Myeongkyu ^{[3
]}

Yun, Ji-Hye ^{[3
]}

Heo, Yunseok ^{[3
]}

Dewilde, Sylvia ^{[4
]}

Shiratani, Masaharu ^{[1
]}

Choi, Eun Ha ^{[5
]}

Lee, Weontae ^{[3
]}

Bogaerts, Annemie ^{[2
]}

机构：

[1] Kyushu Univ, Ctr Plasma Nanointerface Engn, Fukuoka, Japan

[2] Univ Antwerp, Dept Chem, Res Grp PLASMANT, Antwerp, Belgium

[3] Yonsei Univ, Coll Life Sci & Biotechnol, Dept Biochem, 134 Shinchon Dong, Seoul 120749, South Korea

[4] Univ Antwerp, Dept Biomed Sci, Res Grp PPES, Univ Pl 1, B-1610 Antwerp, Belgium

[5] Kwangwoon Univ, Dept Elect & Biol Phys, Seoul 01897, South Korea

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2020年 / 163卷

基金：

新加坡国家研究基金会; 欧盟地平线“2020”;

关键词：

Noxa1; SH3; Oxidative stress; X-ray crystallography; MD simulations; MOLECULAR-DYNAMICS; PLASMA; INACTIVATION; PROTEINS; FAMILY; FOCUS;

D O I：

10.1016/j.ijbiomac.2020.09.120

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

NADPH oxidases 1 (NOX1) derived reactive oxygen species (ROS) play an important role in the progression of cancer through signaling pathways. Therefore, in this paper, we demonstrate the effect of cold atmospheric plasma (CAP) on the structural changes of Noxa1 SH3 protein, one of the regulatory subunits of NOX1. For this purpose, firstly we purified the Noxa1 SH3 protein and analyzed the structure using X-ray crystallography, and subsequently, we treated the protein with two types of CAP reactors such as pulsed dielectric barrier discharge (DBD) and Soft Jet for different time intervals. The structural deformation of Noxa1 SH3 protein was analyzed by various experimental methods (circular dichroism, fluorescence, and NMR spectroscopy) and by MD simulations. Additionally, we demonstrate the effect of CAP (DBD and Soft Jet) on the viability and expression of NOX1 in A375 cancer cells. Our results are useful to understand the structural modification/oxidation occur in protein due to reactive oxygen and nitrogen (RONS) species generated by CAP. (C) 2020 Elsevier B.V. All rights reserved.

引用

页码：2405 / 2414

页数：10

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