In vitro regeneration of Echinacea purpurea from leaf explants

被引：99

作者：

Koroch, A ^{[1
]}

Juliani, HR ^{[1
]}

Kapteyn, J ^{[1
]}

Simon, JE ^{[1
]}

机构：

[1] Rutgers State Univ, Ctr New Use Agr & Nat Plant Prod, New Brunswick, NJ 08901 USA

来源：

PLANT CELL TISSUE AND ORGAN CULTURE | 2002年 / 69卷 / 01期

关键词：

organogenesis; plant regeneration; purple coneflower; tissue culture;

D O I：

10.1023/A:1015042032091

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Efficient plant regeneration was achieved via organogenesis from callus cultures derived from leaf tissue of Echinacea purpurea. Proliferating shoot cultures were obtained by placing leaf explants on Murashige and Skoog (MS) medium supplemented with 6-benzylaminopurine (BAP) and naphthaleneacetic acid (NAA) combinations. MS medium supplemented with BAP (4.44 muM) and NAA (0.054 muM) was the most effective, providing high shoot regeneration frequencies (100%) associated with a high number of shoots per explant (7.7 shoots/explant). Plantlets were rooted on MS medium alone or in combination with different concentrations of indole-3-butyric acid (IBA), and high rooting and survival was achieved using MS media without plant growth regulators (PGR). All plantlets survived acclimatization producing healthy plants in the greenhouse. This study demonstrated that adventitious shoot regeneration of E. purpurea from leaf explants can be a useful method for the multiplication of this important medicinal plant.

引用

页码：79 / 83

页数：5

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