Whole-cell Escherichia coli-based bio-sensor assay for dual zinc oxide nanoparticle toxicity mechanisms

A whole-cell biosensor assay for dual ZnO nanoparticle toxicity mechanisms has been developed based on the transcriptional response of Escherichia colt to: (1) Zn2+ from ZnO nanoparticle dissolution with genes zntA (Zn2+ efflux) and znuABC (Zn2+ uptake); and (2) redox stress from ZnO nanoparticle photo-electron production under ultraviolet light with genes soxS and katG. Both processes occur in a dispersion of ZnO nanoparticles leading to toxicity. ZnO nanoparticle dissolution was measured independently by ICP-MS and photo-radical generation was confirmed by the stochiometric reduction of the redox dye, 2, 6-dichloroindolphenol (DCPIP). The whole-cell biosensor can detect both toxicity mechanisms and is a species-specific assay capable of discriminating between ZnO nanoparticles and the Zn2+ dissolution product (c) 2013 Elsevier B.V. All rights reserved.