Model studies for isolation of G-quadruplex-forming DNA sequences through a pull-down strategy with macrocyclic polyoxazole

被引：3

作者：

Iida, Keisuke ^{[2
]}

Tsushima, Yamato ^{[1
]}

Ma, Yue ^{[1
]}

Masoud, Shadi Sedghi ^{[1
]}

Sakuma, Mai ^{[1
]}

Yokoyama, Tomomi ^{[1
]}

Yoshida, Wataru ^{[3
]}

Ikebukuro, Kazunori ^{[1
]}

Nagasawa, Kazuo ^{[1
]}

机构：

[1] Tokyo Univ Agr & Technol, Dept Biotechnol & Life Sci, 2-24-16 Naka Cho, Koganei, Tokyo 1848588, Japan

[2] Chiba Univ, Dept Chem, Inage Ku, 1-33 Yayoi, Chiba 2638522, Japan

[3] Tokyo Univ Technol, Sch Biosci & Biotechnol, Grad Sch Bion, 1404-1 Katakuramachi, Hachioji, Tokyo 1920982, Japan

来源：

BIOORGANIC & MEDICINAL CHEMISTRY | 2019年 / 27卷 / 08期

基金：

日本学术振兴会;

关键词：

G-quadruplex; Macrocyclic oxazole; Pull-down strategy; Telomestatin; RNA G-QUADRUPLEXES;

D O I：

10.1016/j.bmc.2019.02.056

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

G-quadruplexes (G4s) are non-B DNA structures present in guanine-rich regions of gene regulatory areas, promoters and CpG islands, but their occurrence and functions remain incompletely understood. Thus, methodology to identify G4 sequences is needed. Here, we describe the synthesis of a novel cyclic hepta-oxazole compound, L1Bio-7OTD (1), bearing a biotin affinity-tag as a tool to pull down G4 structures from mixtures of G4-forming and non G4-forming DNA sequences. We confirmed that it could pull down G4s associated with telomeres, bcl-2 gene, and c-kit gene.

引用

页码：1742 / 1746

页数：5

共 8 条

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