Cell sorting in a Petri dish controlled by computer vision

被引：34

作者：

Koernyei, Z. ^{[1
]}

Beke, S. ^{[2
]}

Mihalffy, T. ^{[3
]}

Jelitai, M. ^{[1
]}

Kovacs, K. J. ^{[1
]}

Szabo, Z. ^{[4
]}

Szabo, B. ^{[4
,5
]}

机构：

[1] Hungarian Acad Sci, Inst Expt Med, H-1083 Budapest, Hungary

[2] RIKEN Adv Sci Inst, Laser Technol Lab, Wako, Saitama 3510198, Japan

[3] Furukawa Elect Inst Technol, H-1158 Budapest, Hungary

[4] CellSorter Co Innovat, H-1037 Budapest, Hungary

[5] Eotvos Lorand Univ, Dept Biol Phys, H-1117 Budapest, Hungary

来源：

SCIENTIFIC REPORTS | 2013年 / 3卷

关键词：

SORTER; BAD;

D O I：

10.1038/srep01088

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Fluorescence-activated cell sorting (FACS) applying flow cytometry to separate cells on a molecular basis is a widespread method. We demonstrate that both fluorescent and unlabeled live cells in a Petri dish observed with a microscope can be automatically recognized by computer vision and picked up by a computer-controlled micropipette. This method can be routinely applied as a FACS down to the single cell level with a very high selectivity. Sorting resolution, i.e., the minimum distance between two cells from which one could be selectively removed was 50-70 micrometers. Survival rate with a low number of 3T3 mouse fibroblasts and NE-4C neuroectodermal mouse stem cells was 66 +/- 12% and 88 +/- 16%, respectively. Purity of sorted cultures and rate of survival using NE-4C/NE-GFP-4C co-cultures were 95 +/- 2% and 62 +/- 7%, respectively. Hydrodynamic simulations confirmed the experimental sorting efficiency and a cell damage risk similar to that of normal FACS.

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页数：10

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