Hyperthermia in combination with oxidative stress induces autophagic cell death in HT-29 colon cancer cells

被引：46

作者：

Chen, Fei ^{[1
]}

Wang, Chia-Chi ^{[1
]}

Kim, Eugene ^{[1
]}

Harrison, Lawrence E. ^{[1
]}

机构：

[1] Univ Med & Dent New Jersey, New Jersey Med Sch, Dept Surg, Div Surg Oncol, Newark, NJ 07103 USA

来源：

CELL BIOLOGY INTERNATIONAL | 2008年 / 32卷 / 07期

关键词：

Autophagy; Oxidative stress; Colon cancer;

D O I：

10.1016/j.cellbi.2008.02.010

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The purpose of this study was to evaluate the mechanism of ROS-induced hyperthermic cell death in a colon cancer cell line. HT-29 colon cancer cells were exposed to heat (43 degrees C) in the presence of tert-butyl hydroperoxide (t-BOOH). t-BOOH combined with hyperthermia significantly decreased cell viability as compared with t-BOOH or hyperthermia alone. This decrease in cell numbers was associated with retardation in the S phase transit and not through apoptosis. Cell death was noted to be accompanied by specific features characteristic of autophagy: the presence of cytoplasmic autophagic vacuoles; autophagosome membrane association of microtubule-associated protein light chain 3; accumulation of acidic vesicular organelles; and increased incorporation of MDC in the autophagosome. Thermal sensitization through modulation of cellular ROS may represent a novel approach to increase the efficacy of hyperthermia as an anticancer modality. (C) 2008 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.

引用

页码：715 / 723

页数：9

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