Autoantibodies to 60 kDa SS-A/Ro yield a specific nuclear myriad discrete fine speckled immunofluorescence pattern

被引：11

作者：

Dellavance, Alessandra ^{[1
]}

Alvarenga, Rossana Rassi ^{[2
]}

Rodrigues, Silvia Helena ^{[2
,3
]}

Barbosa, Silvia Helena ^{[3
]}

Pinto Camilo, Amandia Cristina ^{[3
]}

Okamoto Shiguedomi, Herika Santiago ^{[3
]}

Rodrigues, Silvia Sanchez ^{[3
]}

Silva, Cristiane Gallindo ^{[4
]}

Coelho Andrade, Luis Eduardo ^{[2
,3
]}

机构：

[1] Fleury Med & Hlth Labs, Div Res & Dev, Sao Paulo, Brazil

[2] Univ Fed Sao Paulo, UNIFESP, Div Rheumatol, BR-04023900 Sao Paulo, Brazil

[3] Fleury Med & Hlth Labs, Div Immunol, Sao Paulo, Brazil

[4] Fleury Med & Hlth Labs, Reagents Prod Div, Sao Paulo, Brazil

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 2013年 / 390卷 / 1-2期

关键词：

Antinuclear antibodies; Autoantibodies; SS-A/Ro; Systemic lupus erythematosus; Sjogren's syndrome; SYSTEMIC-LUPUS-ERYTHEMATOSUS; GOLGI-COMPLEX; ANTINUCLEAR ANTIBODIES; MOLECULAR CHARACTERIZATION; HEALTHY-INDIVIDUALS; RHEUMATIC-DISEASES; SJOGRENS-SYNDROME; PROTEIN; AUTOANTIGEN; ANTIGENS;

D O I：

10.1016/j.jim.2013.01.006

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Objectives: To report on a novel immunofluorescence pattern specifically associated with antibodies to SS-A/Ro. Methods: A novel immunofluorescence pattern, herein designated SS-A/Ro pattern, was characterized as myriad discrete fine speckles throughout the nucleus. Eighty-six sequential samples presenting the SS-A/Ro pattern and 64 samples presenting non-SS-A/Ro nuclear fine speckled pattern at the ANA-HEp-2 routine were screened for SS-A/Ro reactivity. Conversely, 48 samples with known reactivity to 60 kDa SS-A/Ro, 13 samples with exclusive reactivity to 52 kDa SS-A/Ro, and 48 SS-A/Ro-negative samples were analyzed for the ANA-HEp-2 pattern. Results: Eighty-five of the 86 samples (98.8%) presenting the SS-A/Ro pattern and 15 of the 64 (23.4%) samples with non-SS-A/Ro fine speckled pattern reacted with 60 kDa SS-A/Ro. Conversely, 44 (91.6%) of 48 samples with known reactivity to 60 kDa SS-A/Ro presented the SS-A/Ro pattern and four (8.4%) presented non-SS-A/Ro fine speckled pattern. None of the 48 anti-SS-A/Ro-negative samples and none of anti-52 kDa SS-A/Ro-positive samples yielded the SS-A/Ro pattern. This immunofluorescence pattern was observed in different commercial HEp-2 cell slides and in homemade HEp-2 cell slides. Conclusions: The SS-A/Ro pattern belongs to the group of immunofluorescence patterns that hold strong association with the respective autoantibody specificities, such as those associated with CENP-F and NuMA-1. The identification of the SS-A/Ro pattern at the ANA-HEp-2 screening routine shall lead to specific tests for the identification of anti-SS-A/Ro antibodies. (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：35 / 40

页数：6

共 11 条

[11] Anti-SS-A/Ro antibody determination by indirect immunofluorescence and comparison of different methods of anti-nuclear antibody screening Evalution of the utility of HEp-2 cells transfected with the 60 kDa SS-A/Ro as a substrate
Tanaka, Noriyo
Muro, Yoshinao
Sugiura, Kazumitsu
Tomita, Yasushi
MODERN RHEUMATOLOGY, 2008, 18 (06) : 585 - 592

← 1 2 →