Morphological study of fragmented DNA on touched objects

被引：65

作者：

Kita, Toshiro ^{[1
]}

Yamaguchi, Hiroki ^{[2
]}

Yokoyama, Mitsuru ^{[3
]}

Tanaka, Toshiko ^{[1
]}

Tanaka, Noriyuki

机构：

[1] Univ Occupat & Environm Hlth, Sch Med, Dept Forens Med, Yahata Nishi Ku, Kitakyushu, Fukuoka 8078555, Japan

[2] Fukuoka Prefectural Police Headquartes, Forens Sci Lab, Fukuoka 8128576, Japan

[3] Univ Occupat & Environm Hlth, Bioinformat Res Ctr, Yahata Nishi Ku, Kitakyushu, Fukuoka 8078555, Japan

来源：

FORENSIC SCIENCE INTERNATIONAL-GENETICS | 2008年 / 3卷 / 01期

关键词：

Touch DNA; Genetic profile; DNA fragment; Nuclear DNA; Skin; Keratinization;

D O I：

10.1016/j.fsigen.2008.09.002

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

In recent years, forensic scientists showed that an individual's genetic profile can be retrieved from touched objects. Degraded DNA is believed to originate from epidermal cells and to be responsible for this phenomenon, yet the mechanism has not been confirmed. In the present Study, we carried out a morphological and immunohistochemical investigation of nuclear DNA in differentiating keratinocytes in the skin and also a genetic analysis of DNA on swabs Of human skin. Immunoelectron microscope analysis showed that single-stranded DNA was found both in the cornified layer of the skin and in swabs. Real-time-PCR assay proved that the DNA in the swabs was derived from the human DNA. Electron microscopic analysis of shadow-cast showed the presence of small DNA fragments in the swabs. It is conceivable that these DNA fragments Oil touched objects may originate from the epidermal cells of the cornified layer that are constantly sloughed off and leave for Skin surface with sweat. (c) 2008 Elsevier Ireland Ltd. All rights reserved.

引用

页码：32 / 36

页数：5

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