Variations in the chemical profile and biological activities of licorice (Glycyrrhiza glabra L.), as influenced by harvest times

被引:30
作者
Cheel, Jose [1 ,2 ]
Tumova, Lenka [1 ]
Areche, Carlos [3 ]
Van Antwerpen, Pierre [4 ]
Neve, Jean [4 ]
Zouaoui-Boudjeltia, Karim [5 ]
San Martin, Aurelio [3 ]
Vokral, Ivan [6 ]
Wsol, Vladimir [7 ]
Neugebauerova, Jarmila [8 ]
机构
[1] Charles Univ Prague, Fac Pharm, Dept Pharmacognosy, Hradec Kralove 50005, Czech Republic
[2] Contipro Biotech Sro, Dept Res & Dev, Dolni Dobrouc 56102, Czech Republic
[3] Univ Chile, Fac Ciencias, Dept Quim, Santiago, Chile
[4] Univ Libre Bruxelles, Fac Pharm, Lab Pharmaceut Chem & Analyt Platform, B-1050 Brussels, Belgium
[5] Univ Libre Bruxelles, CHU Charleroi, Expt Med Lab, B-6110 Montignies le Tilleul, Belgium
[6] Charles Univ Prague, Fac Pharm, Dept Pharmacol & Toxicol, Hradec Kralove 50005, Czech Republic
[7] Charles Univ Prague, Fac Pharm, Dept Biochem Sci, Hradec Kralove 50005, Czech Republic
[8] Mendel Univ Brno, Fac Hort, Lednice 69144, Czech Republic
关键词
Licorice; Harvest times; Antioxidant activity; HPLC; Flavonoids; SEASONAL-VARIATIONS; ANTIOXIDANT ACTIVITY; CONSTITUENTS; FLAVONOIDS; ROOTS; MYELOPEROXIDASE; FRUITS; ACID; LDL; URALENSIS;
D O I
10.1007/s11738-012-1174-9
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
This study investigates the variations in the chemical profile, free radical scavenging, antioxidant and gastroprotective activities of licorice extracts (LE) from plants harvested during the months of February to November. Correlations between biological properties and the chemical composition of LE were also investigated. The results showed that the total contents of phenols, flavonoids and tannins in LE varied at different harvest times. Liquiritin and glycyrrhizin, the major components of LE, varied in the range of 28.65-62.80 and 41.84-114.33 mg g(-1), respectively. The relative proportion of glycyrrhizin derivative (3), glabridin (4), glabrene (5) and liquiritigenin derivative (6), varied in the range of 0.88-11.38 %, 1.86-10.03 %, 1.80-18.40 % and 5.53-16.31 %, respectively. These fluctuations correlated positively with changes in the antioxidant and free radical scavenging activities of licorice. In general, the samples from May and November showed the most favorable free radical scavenging and antioxidant effects, whereas the best gastroprotective effect was in May. Liquiritin and glycyrrhizin, the major constituents in the February and May LE, appeared to contribute to the superoxide radical scavenging and gastroprotective effects. Glabridin and glabrene, the compounds with the highest relative proportion in the November LE, accounted for the antioxidant and DPPH scavenging activities of licorice. It is concluded that the chemical profile of licorice quantitatively varied at different harvest times and these fluctuations determined changes in its bioactivities. These data could pave the way to optimize harvesting protocols for licorice in relation with its health-promoting properties.
引用
收藏
页码:1337 / 1349
页数:13
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