Graphene oxide functionalized with chitosan based nanoparticles as a carrier of siRNA in regulating Bcl-2 expression on Saos-2 & MG-63 cancer cells and its inflammatory response on bone marrow derived cells from mice

被引：31

作者：

Saravanabhavan, Shanmuga Sundar ^{[1
,2
]}

Rethinasabapathy, Muruganantham ^{[3
]}

Zsolt, Sarang ^{[2
]}

Kalambettu, Aravind Bhat ^{[4
]}

Elumalai, Sundaravadivel ^{[5
]}

Janakiraman, Manokaran ^{[1
]}

Huh, Yun Suk ^{[3
]}

Natesan, Balasubramanian ^{[1
]}

机构：

[1] Anna Univ, Dept Chem Engn, Chennai 600025, Tamil Nadu, India

[2] Univ Debrecen, Dept Biochem & Mol Biol, Fac Med, H-4010 Debrecen, Hungary

[3] Inha Univ, Dept Biol Engn, BSRC, Incheon 22212, South Korea

[4] Saveetha Dent Coll, Dept Orthodont, Chennai 600077, Tamil Nadu, India

[5] SRM Univ, Kattankulathur 603203, Tamil Nadu, India

来源：

MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS | 2019年 / 99卷

基金：

新加坡国家研究基金会;

关键词：

Inflammation; Bone marrow derived macrophages; RAW macrophages; Graphene oxide; Chitosan; siRNA; Osteosarcoma; IN-VITRO; DELIVERY PLATFORM; FABRICATION; FAMILY; BIOCOMPATIBILITY; CHEMOTHERAPY; NANOFIBER; RELEASE;

D O I：

10.1016/j.msec.2019.02.047

中图分类号：

TB3 [工程材料学]; R318.08 [生物材料学];

学科分类号：

0805 ; 080501 ; 080502 ;

摘要：

Presently, quite a lot of research that are being carried out to find a potential cure for cancer and many had made to clinical trial stage as well. In the present study, we focus on use of a novel graphene oxide functionalized chitosan nanoparticle targeting Saos-2 and MG-63 osteosarcoma cells. The graphene oxide chitosan nano particles were loaded with siRNA, studied for in vitro release with varying concentration & pH, and fitted to peppas model. MIT & ROS assay was used to evaluate biocompatibility of carrier and qPCR to study the inflammatory responses in particular checking gene expression of IL-6, TGF-beta, TNF-alpha in both RAW 264.7 and bone marrow derived macrophages. The results of study showed that release of siRNA were in a controlled fashion and effective at acidic pH that prevails on tumor site. The material was biocompatible and effective in case of Saos-2 osteosarcoma cells with a viability of 0.4 +/- 0.43 and 0.49 +/- 0.53 in case of MG-63 cells when treated with highest concentration of 100 mu l siRNA compared to untreated cells that were in range of 0.64 +/- 0.67 in Saos-2 and 0.61 +/- 0.63 in MG-63 cells. The results of expression of inflammatory cytokines IL-6, TGF-beta & TNF-alpha showed negligible amount compared to control group serving the purpose of an effective carrier targeting tumor cells.

引用

页码：1459 / 1468

页数：10