RETRACTED: Reduced levels of miR-485-5p in HPV-infected cervical cancer promote cell proliferation and enhance invasion ability (Retracted Article)

被引:17
作者
Dai, Yuanyuan [1 ]
Xie, Fengyan [1 ]
Chen, Yan [1 ]
机构
[1] Wenzhou Med Univ, Affiliated Hosp 3, Dept Obstet & Gynecol, 108 Wansong Rd, Wenzhou 325200, Zhejiang, Peoples R China
关键词
cell proliferation; cervical cancer; FLOT-1; HPV-infected; invasion; microRNA-485-5p; POOR-PROGNOSIS; NONCODING RNAS; UP-REGULATION; MICRORNAS; FLOTILLIN-1; METASTASIS; EXPRESSION; PROGRESSION; CARCINOMA; DIAGNOSIS;
D O I
10.1002/2211-5463.12869
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Cervical cancer (CC) is the most common gynecological malignancy, with high incidence and mortality rates in China. The microRNA miR-485-5p has previously been reported to serve as a negative regulator of tumorigenesis in breast cancer and hepatocellular carcinoma, and miR-485-5p has been observed to be differentially expressed between CC and normal control tissue. Here, we confirmed that miR-485-5p expression is lower in CC than in adjacent normal tissue and proceeded to investigate the effects of miR-485 on tumor behavior in CC cell lines. We report that miR-485-5p transcription is decreased in HPV-infected CC tissue, and levels of miR-485 in clinical samples are positively correlated with the 5-year overall survival rate. The Transwell assay showed that miR-485-5p inhibited cell invasion and migration but had no influence on apoptosis and cell proliferation. Using a luciferase reporter assay, we demonstrated that miR-485-5p partially abrogated cell migration and proliferation by targeting FLOT-1 mRNA. Transfection of HPV-infected cervical carcinoma cells with an adenovirus vector encoding human FLOT-1 partially diminished the inhibitory effects of miR-485 on cell invasion. Taken, together, these data demonstrated that miR-485-5p suppresses the invasion of cancer cells by targeting FLOT-1 in HPV-infected cervical carcinoma cells.
引用
收藏
页码:1348 / 1361
页数:14
相关论文
共 37 条
[1]   MicroRNAs as Diagnostic, Prognostic, and Therapeutic Biomarkers in Prostate Cancer [J].
Aghdam, Arad Mobasher ;
Amiri, Atefeh ;
Salarinia, Reza ;
Masoudifar, Aria ;
Ghasemi, Faezeh ;
Mirzaei, Hamed .
CRITICAL REVIEWS IN EUKARYOTIC GENE EXPRESSION, 2019, 29 (02) :127-139
[2]  
[Anonymous], 2018, J CELL BIOCH, DOI DOI 10.1002/jcb.26271
[3]   miRNA-target network reveals miR-124as a key miRNA contributing to clear cell renal cell carcinoma aggressive behaviour by targeting CAV1 and FLOT1 [J].
Butz, Henriett ;
Szabo, Peter M. ;
Khella, Heba W. Z. ;
Nofech-Mozes, Roy ;
Patocs, Attila ;
Yousef, George M. .
ONCOTARGET, 2015, 6 (14) :12543-12557
[4]   Upregulation of flotillin-1 promotes invasion and metastasis by activating TGF-β signaling in nasopharyngeal carcinoma [J].
Cao, Sumei ;
Cui, Yanmei ;
Xiao, Huiming ;
Mai, Miaoqing ;
Wang, Chanjuan ;
Xie, Shanghang ;
Yang, Jing ;
Wu, Shu ;
Li, Jun ;
Song, Libing ;
Guo, Xiang ;
Lin, Chuyong .
ONCOTARGET, 2016, 7 (04) :4252-4264
[5]  
Di Jiangli, 2015, Asian Pac J Cancer Prev, V16, P7401
[6]   MicroRNAs, miR-154, miR-299-5p, miR-376a, miR-376c, miR-377, miR-381, miR-487b, miR-485-3p, miR-495 and miR-654-3p, mapped to the 14q32.31 locus, regulate proliferation, apoptosis, migration and invasion in metastatic prostate cancer cells [J].
Formosa, A. ;
Markert, E. K. ;
Lena, A. M. ;
Italiano, D. ;
Finazzi-Agro, E. ;
Levine, A. J. ;
Bernardini, S. ;
Garabadgiu, A. V. ;
Melino, G. ;
Candi, E. .
ONCOGENE, 2014, 33 (44) :5173-5182
[7]   GD2-targeted immunotherapy and potential value of circulating microRNAs in neuroblastoma [J].
Gholamin, Sharareh ;
Mirzaei, Hamed ;
Razavi, Seyed-Mostafa ;
Hassanian, Seyed Mahdi ;
Saadatpour, Leila ;
Masoudifar, Aria ;
ShahidSales, Soodabeh ;
Avan, Amir .
JOURNAL OF CELLULAR PHYSIOLOGY, 2018, 233 (02) :866-879
[8]   MicroRNAs in retinoblastoma: Potential diagnostic and therapeutic biomarkers [J].
Golabchi, Khodayar ;
Soleimani-Jelodar, Rahim ;
Aghadoost, Nazila ;
Momeni, Fatemeh ;
Moridikia, Abdullah ;
Nahand, Javid Sadri ;
Masoudifar, Aria ;
Razmjoo, Hasan ;
Mirzaei, Hamed .
JOURNAL OF CELLULAR PHYSIOLOGY, 2018, 233 (04) :3016-3023
[9]   Estrogen acidifies vaginal pH by up-regulation of proton secretion via the apical membrane of vaginal-ectocervical epithelial cells [J].
Gorodeski, GI ;
Hopfer, U ;
Liu, CC ;
Margles, E .
ENDOCRINOLOGY, 2005, 146 (02) :816-824
[10]   Overexpression of flotillin-1 is involved in proliferation and recurrence of bladder transitional cell carcinoma [J].
Guan, Yawei ;
Song, Haiyan ;
Zhang, Guohui ;
Ai, Xing .
ONCOLOGY REPORTS, 2014, 32 (02) :748-754