Detection of Listeria Monocytogenes by Electrochemical Impedance Spectroscopy

被引：34
作者：
Radhakrishnan, Rajeswaran [1 ]
Jahne, Michael [2 ]
Rogers, Shane [2 ]
Suni, Ian I. [1 ,3 ]
机构：
[1] Clarkson Univ, Mat Sci & Engn Program, Potsdam, NY 13699 USA
[2] Clarkson Univ, Dept Civil & Environm Engn, Potsdam, NY 13699 USA
[3] Clarkson Univ, Dept Chem & Biomol Engn, Potsdam, NY 13699 USA
来源：
ELECTROANALYSIS | 2013年 / 25卷 / 09期
基金：
美国国家科学基金会;
关键词：
Biosensors; Electrochemical Impedance Spectroscopy; Food Pathogens; Immunoassays; SELF-ASSEMBLED MONOLAYERS; REAL-TIME PCR; RAPID DETECTION; RISK-ASSESSMENT; SURFACES; BIOSENSOR; SENSORS; ARRAYS; GOLD;
D O I：
10.1002/elan.201300140
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Listeria monocytogenes is detected by electrochemical impedance spectroscopy using a mouse monoclonal antibody immobilized onto an Au electrode. This yields sensitivities of 0.825k cm(2)/(CFU/mL) and 1.129k cm(2)/(CFU/mL) and detection limits of 5CFU/mL and 4CFU/mL for ideal solutions and filtered tomato extract, respectively. Control experiments with an Au electrode onto which a mouse monoclonal antibody to GAPDH is immobilized demonstrate that non-specific adsorption is insignificant for the system and methodology studied here. Control experiments with Salmonella enterica demonstrate no cross-reactivity to this food pathogen. Potential technological hurdles to development of a multiplexed impedance biosensor for food pathogens are also discussed.
引用
收藏
页码：2231 / 2237
页数：7
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