Simultaneous measurements of a specimen quantitative-phase signal and its surrounding medium refractive index using quantitative-phase imaging

被引：3
作者：
de Dorlodot, Bertrand [2 ]
Belanger, Erik [2 ,3 ,4 ]
Rioux-Pellerin, Emile [2 ]
Marquet, Pierre [1 ,2 ,4 ]
机构：
[1] Univ Laval, Dept Psychiat & Neurosci, Quebec City, PQ G1V 0A6, Canada
[2] Univ Laval, Ctr Rech CERVO, Quebec City, PQ G1J 2G3, Canada
[3] Univ Laval, Dept Phys Genie Phys & Opt, Quebec City, PQ G1V 0A6, Canada
[4] Univ Laval, Ctr Opt Photon & Laser, Quebec City, PQ G1V 0A6, Canada
来源：
OPTICS LETTERS | 2020年 / 45卷 / 19期
基金：
加拿大自然科学与工程研究理事会; 加拿大创新基金会;
关键词：
DIGITAL HOLOGRAPHIC MICROSCOPY; CELLS;
D O I：
10.1364/OL.391641
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
This Letter demonstrates a method to simultaneously measure the quantitative-phase signal (QPS) of the observed. specimen and the refractive index of its surrounding medium (n(m)) in a time-resolved manner using a micro-structured coverslip. Such coverslips, easily integrated into perfused live-cell imaging chambers, allow to use various quantitative-phase imaging techniques to achieve this dual measurement. Since QPS is crucially dependent on n(m), the measurement of the latter paves the way for its manipulation in a controlled manner leading to a QPS contrast modulation for appealing applications, including visualizing the interior of cells. (C) 2020 Optical Society of America
引用
收藏
页码：5587 / 5590
页数：4
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共 16 条
  • [1] Living cell dry mass measurement using quantitative phase imaging with quadriwave lateral shearing interferometry: an accuracy and sensitivity discussion
    Aknoun, Sherazade
    Savatier, Julien
    Bon, Pierre
    Galland, Frederic
    Abdeladim, Lamiae
    Wattellier, Benoit
    Monneret, Serge
    [J]. JOURNAL OF BIOMEDICAL OPTICS, 2015, 20 (12)
  • [2] Measuring Absolute Cell Volume Using Quantitative-Phase Digital Holographic Microscopy and a Low-Cost, Open-Source, and 3D-Printed Flow Chamber
    Belanger, Erik
    Levesque, Sebastian A.
    Rioux-Pellerin, Emile
    Lavergne, Pauline
    Marquet, Pierre
    [J]. FRONTIERS IN PHYSICS, 2019, 7
  • [3] Comparative study of quantitative phase imaging techniques for refractometry of optical waveguides
    Belanger, Erik
    Berube, Jean-Philippe
    de Dorlodot, Bertrand
    Marquet, Pierre
    Vallee, Real
    [J]. OPTICS EXPRESS, 2018, 26 (13): : 17498 - +
  • [4] A tunable refractive index matching medium for live imaging cells, tissues and model organisms
    Boothe, Tobias
    Hilbert, Lennart
    Heide, Michael
    Berninger, Lea
    Huttner, Wieland B.
    Zaburdaev, Vasily
    Vastenhouw, Nadine L.
    Myers, Eugene W.
    Drechsel, David N.
    Rink, Jochen C.
    [J]. ELIFE, 2017, 6
  • [5] Determination of Transmembrane Water Fluxes in Neurons Elicited by Glutamate Ionotropic Receptors and by the Cotransporters KCC2 and NKCC1: A Digital Holographic Microscopy Study
    Jourdain, Pascal
    Pavillon, Nicolas
    Moratal, Corinne
    Boss, Daniel
    Rappaz, Benjamin
    Depeursinge, Christian
    Marquet, Pierre
    Magistretti, Pierre J.
    [J]. JOURNAL OF NEUROSCIENCE, 2011, 31 (33) : 11846 - 11854
  • [6] Integral refractive index determination of living suspension cells by multifocus digital holographic phase contrast microscopy
    Kemper, Bjoern
    Kosmeier, Sebastian
    Langehanenberg, Patrik
    von Bally, Gert
    Bredebusch, Ilona
    Domschke, Wolfram
    Schnekenburger, Juergen
    [J]. JOURNAL OF BIOMEDICAL OPTICS, 2007, 12 (05)
  • [7] Label-free quantitative cell division monitoring of endothelial cells by digital holographic microscopy
    Kemper, Bjoern
    Bauwens, Andreas
    Vollmer, Angelika
    Ketelhut, Steffi
    Langehanenberg, Patrik
    Muething, Johannes
    Karch, Helge
    von Bally, Gert
    [J]. JOURNAL OF BIOMEDICAL OPTICS, 2010, 15 (03)
  • [8] Automated three-dimensional tracking of living cells by digital holographic microscopy
    Langehanenberg, Patrik
    Ivanova, Lyubomira
    Bernhardt, Ingolf
    Ketelhut, Steffi
    Vollmer, Angelika
    Dirksen, Dieter
    Georgiev, Georgi
    von Bally, Gert
    Kemper, Bjoern
    [J]. JOURNAL OF BIOMEDICAL OPTICS, 2009, 14 (01)
  • [9] Sample and substrate preparation for exploring living neurons in culture with quantitative-phase imaging
    Levesque, Sebastien A.
    Mugnes, Jean-Michel
    Belanger, Erik
    Marquet, Pierre
    [J]. METHODS, 2018, 136 : 90 - 107
  • [10] High-speed interferometric imaging reveals dynamics of neuronal deformation during the action potential
    Ling, Tong
    Boyle, Kevin C.
    Zuckerman, Valentina
    Flores, Thomas
    Ramakrishnan, Charu
    Deisseroth, Karl
    Palanker, Daniel
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2020, 117 (19) : 10278 - 10285
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