Global and gene specific DNA methylation changes during zebrafish development

被引：67

作者：

Fang, Xiefan ^{[1
,2
]}

Corrales, Jone ^{[1
,2
]}

Thornton, Cammi ^{[1
,2
]}

Scheffler, Brian E. ^{[3
]}

Willett, Kristine L. ^{[1
,2
]}

机构：

[1] Univ Mississippi, Dept Pharmacol, Sch Pharm, University, MS 38677 USA

[2] Univ Mississippi, Environm Toxicol Res Program, Sch Pharm, University, MS 38677 USA

[3] USDA ARS, Genom & Bioinformat Res Unit, Stoneville, MS 38776 USA

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 2013年 / 166卷 / 01期

关键词：

DNA methylation; zebrafish; development; vasa; rassf1; EPIGENETIC REGULATION; CPG-ISLANDS; C-JUN; GENOME; EMBRYO; DEMETHYLATION; ACTIVATION; DECREASES; IMPACT; REGION;

D O I：

10.1016/j.cbpb.2013.07.007

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

DNA methylation is dynamic through the life of an organism. Previous studies have primarily focused on DNA methylation changes during very early embryogenesis. In this study, global and gene specific DNA methylation in zebrafish (Danio rerio) embryos, larvae and adult livers were compared. The percent methylation of cytosines was low in 2 to 4.3 h post fertilization (hpf) zebrafish embryos and was consistently higher in zebrafish older than 6 hpf. Furthermore, quantitative real-time PCR (qPCR) results showed relatively high DNA methyltransferase 1 (dnmt1) and low glycine N-methyltransferase (gnmt) mRNA expression in early embryogenesis. By studying methylation patterns and gene expression of five developmentally important genes, namely vase, Ras-association domain family member 1 (rassf1), telomerase reverse transcriptase (tert), c-jun and c-myca, we found that the timing of changes in DNA methylation patterns was gene specific, and changes in gene expression were not necessarily correlated with the DNA methylation patterns. (C) 2013 Elsevier Inc. All rights reserved.

引用

页码：99 / 108

页数：10

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