X-Ray Absorption Spectroscopy Measurements of Cu-ProIAPP Complexes at Physiological Concentrations

被引:5
作者
De Santis, Emiliano [1 ,2 ]
Shardlow, Emma [3 ]
Stellato, Francesco [2 ]
Proux, Olivier [4 ]
Rossi, Giancarlo [1 ,2 ,5 ,6 ]
Exley, Christopher [3 ]
Morante, Silvia [1 ,2 ]
机构
[1] Univ Roma Tor Vergata, Dipartimento Fis, Via Ric Sci, I-00133 Rome, Italy
[2] INFN, Sez Roma 2,Via Ric Sci, I-00133 Rome, Italy
[3] Keele Univ, Lennard Jones Labs, Birchall Ctr, Keele ST5 5BG, Staffs, England
[4] Univ Grenoble Alpes, UMS CNRS 832, Observ Sci Univers Grenoble, F-38041 Grenoble, France
[5] Ctr Fermi Museo Stor Fis, I-00184 Rome, Italy
[6] Ctr Studi & Ric Enrico Fermi, I-00184 Rome, Italy
来源
CONDENSED MATTER | 2019年 / 4卷 / 01期
关键词
X-ray absorption spectroscopy; amylin; high energy resolution fluorescence detection; ISLET AMYLOID POLYPEPTIDE; CROSS-MODULATION; AGGREGATION; PEPTIDE; ZINC; DIFFRACTION; FIBRILS; BINDING; IAPP; SITE;
D O I
10.3390/condmat4010013
中图分类号
O469 [凝聚态物理学];
学科分类号
070205 ;
摘要
The amyloidogenic islet amyloid polypeptide (IAPP) and the associated pro-peptide ProIAPP(1-48) are involved in cell death in type 2 diabetes mellitus. It has been observed that interactions of this peptide with metal ions have an impact on the cytotoxicity of the peptides as well as on their deposition in the form of amyloid fibrils. In particular, Cu(II) seems to inhibit amyloid fibril formation, thus suggesting that Cu homeostasis imbalance may be involved in the pathogenesis of type 2 diabetes mellitus. We performed X-ray Absorption Spectroscopy (XAS) measurements of Cu(II)-ProIAPP complexes under near-physiological (10 mu M), equimolar concentrations of Cu(II) and peptide. Such low concentrations were made accessible to XAS measurements owing to the use of the High Energy Resolved Fluorescence Detection XAS facility recently installed at the ESRF beamline BM16 (FAME-UHD). Our preliminary data show that XAS measurements at micromolar concentrations are feasible and confirm that ProIAPP(1-48)-Cu(II) binding at near-physiological conditions can be detected.
引用
收藏
页码:1 / 8
页数:8
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