Complement modulatory activity of bisbenzylisoquinoline alkaloids isolated from Isopyrum thalictroides -: I Influence on classical pathway in human serum

被引:10
作者
Ivanovska, N [1 ]
Nikolova, P
Hristova, M
Philipov, S
Istatkova, R
机构
[1] Bulgarian Acad Sci, Inst Microbiol, Sofia 1113, Bulgaria
[2] Bulgarian Acad Sci, Inst Organ Chem, Ctr Phytochem, Sofia 1113, Bulgaria
来源
INTERNATIONAL JOURNAL OF IMMUNOPHARMACOLOGY | 1999年 / 21卷 / 05期
关键词
bisbenzylisoquinoline; CP complement activity; Cl-4; activity;
D O I
10.1016/S0192-0561(99)00014-4
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Eleven bisbenzylisoquinoline alkaloids (BBI) were isolated from the plant Isopyrum thalictroides (L.). Treatment of normal human serum (NHS) with BBI resulted in a diminution of the haemolytic activity of the classical pathway (CP). The mode of action of the main alkaloids isopyruthaline (It1), fangchinoline (It2) and isotalictrine (It3) on CP activation was investigated in vitro. The inhibition was time- and temperature-related and for It1 and It3 depended on the concentration of Ca2+ and Mg2+ ions. It was established that the substances reduced C1 haemolytic activity. It2 and It3 enhanced the complement consumption caused by heat aggregated human IgG (HAGG). The BBI prevented the formation of C3 convertase of the classical pathway. The loss of haemolytic activity was partially restored by the addition of C142 reagent (zymosan-treated guinea pig serum) to alkaloids-treated NHS. The addition of the late components C3-9 (EDTA-treated rat sera) recovered to some extent the haemolytic activity of It1-treated NHS, but not of It2- and It3-treated NHS. (C) 1999 International Society for Immunopharmacology. Published by Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:325 / 336
页数:12
相关论文
共 21 条
  • [1] Effect of berbamine on cytosolic phospholipase A(2) activation in rabbit platelets
    Akiba, S
    Nagatomo, R
    Ishimoto, T
    Sato, T
    [J]. EUROPEAN JOURNAL OF PHARMACOLOGY-MOLECULAR PHARMACOLOGY SECTION, 1995, 291 (03): : 343 - 350
  • [2] Bezakova L, 1996, PHARMAZIE, V51, P758
  • [3] The bisbenzylisoquinoline alkaloids, tetrandine and fangchinoline, enhance the cytotoxicity of multidrug resistance-related drugs via modulation of P-glycoprotein
    Choi, SU
    Park, SH
    Kim, KH
    Choi, EJ
    Kim, S
    Park, WK
    Zhang, YH
    Kim, HS
    Jung, NP
    Lee, CO
    [J]. ANTI-CANCER DRUGS, 1998, 9 (03) : 255 - 261
  • [4] Bisbenzylisoquinolines as modulators of chloroquine resistance in Plasmodium falciparum and multidrug resistance in tumor cells
    Frappier, F
    Jossang, A
    Soudon, J
    Calvo, F
    Rasoanaivo, P
    RatsimamangaUrverg, S
    Saez, J
    Schrevel, J
    Grellier, P
    [J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1996, 40 (06) : 1476 - 1481
  • [5] Ivanovska N., 1997, Fitoterapia, V68, P417
  • [6] Antimicrobial and immunological activity of ethanol extracts and fractions from Isopyrum thalictroides
    Ivanovska, N
    Philipov, S
    Istatkova, R
    Georgieva, P
    [J]. JOURNAL OF ETHNOPHARMACOLOGY, 1996, 54 (2-3) : 143 - 151
  • [7] VASODILATOR EFFECTS OF BISBENZYLISOQUINOLINE ALKALOIDS FROM STEPHANIA-CEPHARANTHA
    KAMIYA, T
    SUGIMOTO, Y
    YAMADA, Y
    [J]. PLANTA MEDICA, 1993, 59 (05) : 475 - 476
  • [8] RECONSTRUCTION OF ANTILEPROSY DRUG DEPLETED COMPLEMENT HEMOLYTIC-ACTIVITY BY ADDITION OF ZYMOSAN-TREATED SERA (A SOURCE OF C142) AND CRATEDTA (A SOURCE OF C3-C9)
    KASHYAP, A
    SAHA, K
    SEHGAL, VN
    [J]. INTERNATIONAL JOURNAL OF IMMUNOPHARMACOLOGY, 1992, 14 (08): : 1409 - 1414
  • [9] MICRO-ASSAY FOR COLORIMETRIC ESTIMATION OF COMPLEMENT ACTIVITY IN GUINEA-PIG, HUMAN AND MOUSE SERUM
    KLERX, JPAM
    BEUKELMAN, CJ
    VANDIJK, H
    WILLERS, JMN
    [J]. JOURNAL OF IMMUNOLOGICAL METHODS, 1983, 63 (02) : 215 - 220
  • [10] KONDO Y, 1992, INT J IMMUNOPHARMACO, V14, P1181