Synthesis and spectroscopic characterization of new sulfanilamide-functionalized magnetic nanoparticles, and the usability for carbonic anhydrase purification: is there perspective for 'cancer treatment' application?

被引:5
作者
Bijari, Nooshin [1 ,2 ]
Falsafi, Monireh [3 ]
Pouraghajan, Khadijeh [4 ]
Khodarahmi, Reza [1 ]
机构
[1] Kermanshah Univ Med Sci, Med Biol Res Ctr MBRC, Hlth Technol Inst, Kermanshah, Iran
[2] Semnan Univ, Fac Basic Sci, Dept Biol, Semnan, Iran
[3] Razi Univ, Fac Chem, Dept Inorgan Chem, Kermanshah, Iran
[4] Razi Univ, Fac Sci, Dept Biol, Kermanshah, Iran
关键词
Magnetic nanoparticles; human carbonic anhydrase II; sulfanilamide; protein purification; AFFINITY GEL; ISOZYME-II; THERAPY; BINDING; IMMOBILIZATION; APOPTOSIS; CATALYST; SOLVENT; ALBUMIN; DOCKING;
D O I
10.1080/07391102.2020.1805360
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
This investigation indicated an efficient procedure to purify human carbonic anhydrase II (hCA II) enzyme through sulfanilamide-functionalized (gamma-Fe2O3-CPTES-SA) magnetic nanoparticles (MNPs), where synthesis of Fe(3)O(4)MNPs was carried out using co-precipitation reaction. Next, 3-chloropropyltriethoxysilane (CPTES) was used to modify Fe(3)O(4)nanoparticles and lastly, the surface of the nanoparticles was functionalized with sulfanilamide (SA) as a carbonic anhydrase ligand/inhibitor for binding to hCA II. The characterization of the synthesized nanoparticles was performed using various techniques. These characterization methods revealed that the MNPs were effectively coated with CPTES and SA, and the average diameter of the nanoparticles was approximately 21 nm. The possibility of interaction of gamma-Fe2O3-CPTES-SA nanoparticles with hCA II was studied via multi-spectroscopic techniques. The protein isolated by this single-step procedure had high purity as confirmed by a single band on SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) which was also active after purification. By focusing on their drug loading capacity, and increasing their specificity and affinity to target CA-expressing cancer cells, the synthesized MNPs may dramatically impact the treatment of cancer and become suitable for clinical use in the near future. Communicated by Ramaswamy H. Sarma
引用
收藏
页码:7093 / 7106
页数:14
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