Flow-injection chemiluminescence analysis of lomefloxacin in drugs and biological fluids

A new method was developed for the determination of lomefloxacin by using flow-injection chemiluminescence(CL) based on the reaction of Ce(IV)-sodium thiosulfate-lomefloxacin-sulfuric acid system. The CL conditions were investigated and optimized. The CL intensity was correlated linearly with concentration of lomefloxacin in the range of 1.0x10(-5)similar to 6.0x10(-2) g L-1. The determination limit (3 sigma) is 3x10(-6) g L-1. The relative standard deviation is 1.9% for 11 measurements of 2.0x10(-4) g L-1 lomefloxacin. The method has been applied satisfactorily to determine lomefloxacin in drugs, serum and urine with the recovery of 97.0%similar to 104.5%. The CL mechanism of the system was proposed.