PERFORMANCE CHARACTERISTICS OF HIGH PERFORMANCE LIQUID CHROMATOGRAPHY, FIRST ORDER DERIVATIVE UV SPECTROPHOTOMETRY AND BIOASSAY FOR FLUCONAZOLE DETERMINATION IN CAPSULES

被引：10

作者：

Ruela Correa, Josilene Chaves ^{[1
]}

Reichman, Camila ^{[1
]}

Nunes Salgado, Herida Regina ^{[1
]}

Vianna-Soares, Cristina Duarte ^{[2
]}

机构：

[1] Univ Estadual Paulista, Dept Farmacos & Medicamentos, Fac Ciencias Farmaceut, BR-14801902 Araraquara, SP, Brazil

[2] Univ Fed Minas Gerais, Fac Farm, Dept Prod Farmaceut, BR-31270901 Belo Horizonte, MG, Brazil

来源：

QUIMICA NOVA | 2012年 / 35卷 / 03期

基金：

巴西圣保罗研究基金会;

关键词：

fluconazole; liquid chromatography; bioassay; QUANTITATIVE-DETERMINATION;

D O I：

10.1590/S0100-40422012000300017

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The bioassay, first order derivative UV spectrophotometry and chromatographic methods for assaying fluconazole capsules were compared. They have shown great advantages over the earlier published methods. Using the first order derivative, the UV spectrophotometry method does not suffer interference of excipients. Validation parameters such as linearity, precision, accuracy, limit of detection and limit of quantitation were determined. All methods were linear and reliable within acceptable limits for antibiotic pharmaceutical preparations being accurate, precise and reproducible. The application of each method as a routine analysis should be investigated considering cost, simplicity, equipment, solvents, speed, and application to large or small workloads.

引用

页码：530 / 534

页数：5

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