MicroRNA-27a functions as an oncogene in gastric adenocarcinoma by targeting prohibitin

被引：306

作者：

Liu, Tao ^{[1
]}

Tang, Hua ^{[1
]}

Lang, Yuanyuan ^{[1
]}

Liu, Min ^{[1
]}

Li, Xin ^{[1
]}

机构：

[1] Tianjin Med Univ, Tianjin Life Sci Res Ctr, Tianjin 300070, Peoples R China

来源：

CANCER LETTERS | 2009年 / 273卷 / 02期

关键词：

MicroRNA-27a; Oncogene; Prohibitin; Gastric adenocarcinoma; HUMAN BREAST-CANCER; EXPRESSION PATTERNS; GENES; PROTEIN; CELLS; IDENTIFICATION; DEREGULATION; SPECIFICITY; MICROARRAY; PROFILES;

D O I：

10.1016/j.canlet.2008.08.003

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

MicroRNAs (miRNAs) may function as oncogenes or tumor suppressors. Here, we show that miR-27a is up-regulated in human gastric adenocarcinoma. Suppression of miR-27a inhibits gastric cancer cell growth. Subsequently, prohibitin is identified as a potential miR-27a target, combining bioinformatics and microarray analysis. EGFP report experiment also confirms that the 3' untranslated region (3'UTR) of prohibitin carries the directly binding site of miR-27a. After knockdown of miR-27a in gastric cancer cells, mRNA level and protein level of prohibitin are both elevated. Down-regulation of prohibitin by miR-27a may explain why suppression of miR-27a can inhibit gastric cancer cell growth, further supporting that miR-27a functions as an oncogene. (C) 2008 Elsevier Ireland Ltd. All rights reserved.

引用

页码：233 / 242

页数：10

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