Porcine phosphotyrosine interaction domain containing 1 modulates 3T3-L1 preadipocyte proliferation and differentiation

被引：9

作者：

Chen, Xiaoling ^{[1
]}

Huang, Zhiqing ^{[1
]}

Wang, Huan ^{[1
]}

Jia, Gang ^{[1
]}

Liu, Guangmang ^{[1
]}

Zhao, Hua ^{[1
]}

Nie, Hao ^{[1
]}

机构：

[1] Sichuan Agr Univ, Inst Anim Nutr, Key Lab Anim Dis Resistance Nutr, China Minist Educ, Chengdu 611130, Sichuan, Peoples R China

来源：

BIOLOGIA | 2013年 / 68卷 / 05期

基金：

中国国家自然科学基金;

关键词：

porcine PID1; 3T3-L1; preadipocyte; proliferation; differentiation; FATTY-ACID SYNTHASE; GENE-EXPRESSION; CELL-LINE; NYGGF4; ADIPOCYTES; METABOLISM; PCR;

D O I：

10.2478/s11756-013-0240-0

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The phosphotyrosine interaction domain containing 1 (PID1) gene was firstly isolated from obese subjects and involved in obesity-associated insulin resistance. In the present study, DurocxLandracexYorkshire (DLY) pig PID1 cDNA was cloned. The entire open reading frame of the cloned porcine PID1 is 654 bp. The predicted protein is composed of 217 amino acids residues with a molecular mass of 24,774 Da. Over-expression of porcine PID1 significantly accelerated the proliferation of 3T3-L1 preadipocyte, but inhibited preadipocyte differentiation by decreasing the numerous fat droplets appeared and down-regulating the mRNA expression levels of peroxisome proliferators-activated receptor-gamma, CCAAT/enhancer binding protein alpha, fat acid synthase and lipoprotein lipase. Together, these results suggest that porcine PID1 plays a role in regulating adipose development.

引用

页码：1010 / 1014

页数：5

共 22 条

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