Design, synthesis, and characterization of a calcium-sensitive near infrared dye

被引：20

作者：

Ellis, AL ^{[1
]}

Mason, JC ^{[1
]}

Lee, HW ^{[1
]}

Strekowski, L ^{[1
]}

Patonay, G ^{[1
]}

Choi, H ^{[1
]}

Yang, JJ ^{[1
]}

机构：

[1] Georgia State Univ, Dept Chem, Ctr Drug Design & Adv Biotechnol, Atlanta, GA 30303 USA

来源：

TALANTA | 2002年 / 56卷 / 06期

基金：

美国国家科学基金会;

关键词：

calcium binding; near infrared; benzo-15-crown-5; cyanine dye;

D O I：

10.1016/S0039-9140(01)00641-5

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Intracellular calcium concentration in biological cells varies from 0.1 to 10 muM depending upon cell signaling and disease states. A direct estimate of calcium concentration in cell tissues within this range is possible with a novel calcium-selective reagent 15C5-774. The molecule of 15C5-774 consists of a near-infrared (NIR) chromophore (lambda(max) = 774 nm) and a metal complexing moiety of benzo-15-crown-5. The reagent shows a strong calcium binding affinity in a 1:1 ratio and metal selectivity in the order Ca2+ > Mg2+ > Sr2+ approximate to K+ approximate to Na+ > Zn2+ > Li+. The high sensitivity is achieved by conducting absorption measurements in the NIR region where background interference from the biological matrix is low. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：1099 / 1107

页数：9

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