Design, synthesis, and characterization of a calcium-sensitive near infrared dye

被引：20

作者：

Ellis, AL ^{[1
]}

Mason, JC ^{[1
]}

Lee, HW ^{[1
]}

Strekowski, L ^{[1
]}

Patonay, G ^{[1
]}

Choi, H ^{[1
]}

Yang, JJ ^{[1
]}

机构：

[1] Georgia State Univ, Dept Chem, Ctr Drug Design & Adv Biotechnol, Atlanta, GA 30303 USA

来源：

TALANTA | 2002年 / 56卷 / 06期

基金：

美国国家科学基金会;

关键词：

calcium binding; near infrared; benzo-15-crown-5; cyanine dye;

D O I：

10.1016/S0039-9140(01)00641-5

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Intracellular calcium concentration in biological cells varies from 0.1 to 10 muM depending upon cell signaling and disease states. A direct estimate of calcium concentration in cell tissues within this range is possible with a novel calcium-selective reagent 15C5-774. The molecule of 15C5-774 consists of a near-infrared (NIR) chromophore (lambda(max) = 774 nm) and a metal complexing moiety of benzo-15-crown-5. The reagent shows a strong calcium binding affinity in a 1:1 ratio and metal selectivity in the order Ca2+ > Mg2+ > Sr2+ approximate to K+ approximate to Na+ > Zn2+ > Li+. The high sensitivity is achieved by conducting absorption measurements in the NIR region where background interference from the biological matrix is low. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：1099 / 1107

页数：9

共 37 条

[1] A squaraine-based near IR fluorescent chemosensor for calcium. [J].

Akkaya, EU ;

Turkyilmaz, S .

TETRAHEDRON LETTERS, 1997, 38 (25) :4513-4516

[2]

[Anonymous], METAL IONS BIOL SYST

[3] Formation and crystal structures of novel seven-coordinate 15-crown-5 complexes of manganese(II), iron(II) and cobalt(II) [J].

Atwood, JL ;

Junk, PC .

POLYHEDRON, 2000, 19 (01) :85-91

[4]

BAGSHAW CR, 1987, SPECTROPHOTOMETRY SP

[5] Experimental evidence for alkali-metal ion cation-π interactions using bibracchial lariat ether complexes [J].

Barbour, LJ ;

De Wall, SL ;

Meadows, ES ;

Gokel, GW .

INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH, 2000, 39 (10) :3436-3441

[6] Synthesis and properties of 5-chloro-8-hydroxyquinoline-substituted azacrown ethers: A new family of highly metal ion-selective lariat ethers [J].

Bordunov, AV ;

Bradshaw, JS ;

Zhang, XX ;

Dalley, NK ;

Kou, XL ;

Izatt, RM .

INORGANIC CHEMISTRY, 1996, 35 (25) :7229-7240

[7] Two novel lithium - 15-crown-5 complexes: An extended LiCl chain stabilized by crown ether and a dimeric complex stabilized by hydrogen bonding with water [J].

Boulatov, R ;

Du, B ;

Meyers, EA ;

Shore, SG .

INORGANIC CHEMISTRY, 1999, 38 (20) :4554-4558

[8] Crown ethers: The search for selective ion ligating agents [J].

Bradshaw, JS ;

Izatt, RM .

ACCOUNTS OF CHEMICAL RESEARCH, 1997, 30 (08) :338-345

[9] CALCIUM SIGNALING [J].

CLAPHAM, DE .

CELL, 1995, 80 (02) :259-268

[10] Cation recognition with fluorophore crown ethers [J].

Erk, C .

INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH, 2000, 39 (10) :3582-3588

← 1 2 3 4 →