Gold Nanocrystal Labeling Allows Low-Density Lipoprotein Imaging from the Subcellular to Macroscopic Level

被引：71

作者：

Allijn, Iris E. ^{[1
,2
]}

Leong, Wei ^{[1
]}

Tang, Jun ^{[1
]}

Gianella, Anita ^{[1
]}

Mieszawska, Aneta J. ^{[1
]}

Fay, Francois ^{[1
]}

Ma, Ge ^{[3
]}

Russell, Stewart ^{[4
]}

Callo, Catherine B. ^{[1
]}

Gordon, Ronald E. ^{[5
]}

Korkmaz, Emine ^{[6
]}

Post, Jan Andries ^{[6
]}

Zhao, Yiming ^{[7
]}

Gerritsen, Hans C. ^{[7
]}

Thran, Axel ^{[8
]}

Proksa, Roland ^{[8
]}

Daerr, Heiner ^{[8
]}

Storm, Gert ^{[2
,9
]}

Fuster, Valentin ^{[1
]}

Fisher, Edward A. ^{[4
]}

Fayad, Zahi A. ^{[1
]}

Mulder, Willem J. M. ^{[1
,10
]}

Cormode, David P. ^{[1
,11
]}

机构：

[1] Icahn Sch Med Mt Sinai, Translat & Mol Imaging Inst, New York, NY 10029 USA

[2] Univ Twente, MIRA Inst, NL-7522 NB Enschede, Netherlands

[3] Mt Sinai Sch Med, Dept Oncol Sci, New York, NY 10029 USA

[4] NYU, Langone Med Ctr, New York, NY 10012 USA

[5] Mt Sinai Hosp, Dept Pathol, New York, NY 10029 USA

[6] Univ Utrecht, NL-3512 JE Utrecht, Netherlands

[7] Univ Utrecht, Debye Inst, NL-3512 JE Utrecht, Netherlands

[8] Univ Utrecht, Philips Res Europe, NL-3512 JE Utrecht, Netherlands

[9] Univ Utrecht, Dept Pharmaceut Sci, NL-3512 JE Utrecht, Netherlands

[10] Univ Amsterdam, Acad Med Ctr, Dept Vasc Med, NL-1012 WX Amsterdam, Netherlands

[11] Univ Penn, Perelman Sch Med, Dept Radiol, Philadelphia, PA 19104 USA

来源：

ACS NANO | 2013年 / 7卷 / 11期

基金：

美国国家卫生研究院;

关键词：

low-density lipoprotein; gold nanoparticles; electron microscopy; computed tomography; fluorescence imaging; ATHEROSCLEROTIC LESIONS; APOLIPOPROTEIN B-100; IN-VITRO; NANOPARTICLES; CELLS; MACROPHAGES; THERAPY; CT; RECEPTORS; MECHANISM;

D O I：

10.1021/nn403258w

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Low-density lipoprotein (LDL) plays a critical role in cholesterol transport and is closely linked to the progression of several diseases. This motivates the development of methods to study LDL behavior from the microscopic to whole-body level. We have developed an approach to efficiently load LDL with a range of diagnostically active nanocrystals or hydrophobic agents. We performed focused experiments on LDL labeled with gold nanocrystals (Au-LDL). The labeling procedure had minimal effect on LDL size, morphology, or composition. Biological function was found to be maintained from both in vitro and in vivo experiments. Tumor-bearing mice were injected intravenously with LDL, DIR-LDL, Au-LDL, or a gold-loaded nanoemulsion. LDL accumulation in the tumors was detected with whole-body imaging methods, such as computed tomography (CT), spectral CT, and fluorescence imaging. Cellular localization was studied with transmission electron microscopy and fluorescence techniques. This LDL labeling procedure should permit the study of lipoprotein biointeractions in unprecedented detail.

引用

页码：9761 / 9770

页数：10

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